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- Secondary antibodies
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Product name
Anti-MUSK antibody
See all MUSK products (6) ...
Description
Rabbit polyclonal to MUSK
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH, corresponding to amino acids 844-859 of Human MUSK.
Positive control
Placenta cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
Primary antibody notes
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000.ELISA: 1/1000.
WB: 1/100 - 1/500. Detects a band of approximately 97 kDa (predicted molecular weight: 104 kDa).
IHC-P: 1/50 - 1/100.
Target
Function
Receptor tyrosine kinase that is a key mediator of agrin's action and is involved in neuromuscular junction (NMJ) organization.
Involvement in disease
Defects in MUSK is a cause of congenital myasthenic syndrome with acetylcholine receptor deficiency (CMS-ACHRD) [MIM:608931]. A post-synaptic congenital myasthenic syndrome. Mutations underlying AChR deficiency cause a 'loss of function' and show recessive inheritance. Note=MUSK mutations lead to decreased agrin-dependent AChR aggregation, a critical step in the formation of the neuromuscular junction.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 1 FZ (frizzled) domain.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
Post-translational
modifications
Ubiquitinated by PDZRN3. Ubiquitination promotes endocytosis and lysosomal degradation.
Cellular localization
Membrane.
Target information above from: UniProt accessionO15146
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- skeletal receptor tyrosine-protein kinase antibody
- MDK 4 antibody
- MDK4 antibody
see all
Database links
- Entrez Gene: 4593 Human
- Omim: 601296 Human
- SwissProt: O15146 Human
- Unigene: 156465 Human
- Unigene: 521653 Human
Anti-MUSK antibody images:
Western blot - MUSK antibody (ab5510)
Predicted band size : 104 kDa
ab5510 at a 1/100 dilution staining approximately 97kDa of MUSK in placenta cell lysate by Western blot (ECL).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MUSK antibody (ab5510)
ab5510 staining MUSK in human breast carcinoma (BC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
References for Anti-MUSK antibody (ab5510)
ab5510 has not yet been referenced specifically in any publications.
Publishing research using ab5510? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"