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Anti-MVP antibody [LMR5] (ab3374)

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Overview

Product name

Anti-MVP antibody [LMR5]
See all MVP products (11) ...

Description

Rat monoclonal [LMR5] to MVP

Tested applications

Flow Cyt, IHC-P, IHC-Frmore details

Cross reactivity

Reacts with

Human

Immunogen

Tissue / cell preparation; 2R120 cell line, derived by stepwise Doxorubicin exposure of the SW-1573 non-small cell lung carcinoma cell line.

Positive control

Positive tissue - Cell Lines: Drug-sensitive parental cell lines and their multidrugresistant derivatives. Negative control antibody: Normal rat serum.

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 1% BSA

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

LMR5

Isotype

IgG

Research areas

Cancer >> Drug resistance >> Vault proteins
Signal Transduction >> Protein Trafficking >> Nuclear Import / Export

Applications

Show applications key

Our Abpromise guarantee covers the use of ab3374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Flow Cyt

     

  • IHC-P

     

  • IHC-Fr

     

Application notes

Flow Cyt: use at a diluton of 1/20 - 1/30. Cell permeabilization required.
IHC-P: use at a diluton of 1/20 - 1/30.
IHC-Fr: use at a diluton of 1/20 - 1/30.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.

Tissue specificity

Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.

Sequence similarities

Contains 9 MVP (vault) repeats.

Domain

MVP 3 mediates interaction with PTEN.
MVP 4 mediates interaction with PARP4.

Post-translational
modifications

Phosphorylated on Tyr residues after EGF stimulation.
Dephosphorylated by PTPN11.

Cellular localization

Cytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment.

Target information above from: UniProt accessionQ14764 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 2310009M24Rik antibody
  • LRP antibody
  • Lung Resistance Related Protein antibody
  • Lung resistance-related protein antibody
  • Major Vault Protein antibody
  • Major Vault Protein antibody
  • MVP antibody
  • MVP antibody
  • MVP_HUMAN antibody
  • VAULT 1 antibody
  • VAULT1 antibody
see all

Database links

References for Anti-MVP antibody [LMR5] (ab3374)

This product has been referenced in:

  • Mayr D  et al. Immunohistochemical analysis of drug resistance-associated proteins in ovarian carcinomas. Pathol Res Pract 196:469-75 (2000).Read more (PubMed: 10926324) »
  • Schroeijers AB  et al. Immunohistochemical detection of the human major vault protein LRP with two monoclonal antibodies in formalin-fixed, paraffin-embedded tissues. Am J Pathol 152:373-8 (1998).Read more (PubMed: 9466563) »

See all 2 publications for this product

Publishing research using ab3374? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"