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Overview

  • Product nameAnti-MVP antibodySee all MVP primary antibodies ...
  • Description
    Rabbit polyclonal to MVP
  • Tested applicationsIHC-Fr, WB, IHC-P more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Cat
  • Immunogen

    Recombinant fragment: EVV EIIQATIIRQ NQALRLRARK ECWDRDGKER VTGEEWLVTT VGAYLPAVFE EVLDLVDAVI LTEKTALHLR ARRNFRDFRG VSRRTGEEWL VTVQDTEAHV PDVHEEVLGV VPITTLGPHN YCVILDPVGP DGKNQLGQKR VVKGEKSFFL QPGEQLEQGI QDVYVLSEQQ GLLLRALQPL EEGEDEEKVS, corresponding to amino acids 158-350 of Human MVP (AAH15623)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control293T, A431, H1299, HeLa, HepG2, MOLT4, A549 and Raji cells

Properties

Applications

Our Abpromise guarantee covers the use of ab97311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-Fr IHC-Fr: Use at an assay dependent concentration. PubMed: 22125622
WB WB: 1/500 - 1/3000. Predicted molecular weight: 99 kDa.
IHC-P IHC-P: 1/100 - 1/500.

Target

  • FunctionRequired for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
  • Tissue specificityPresent in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.
  • Sequence similaritiesContains 9 MVP (vault) repeats.
  • DomainMVP 3 mediates interaction with PTEN.
    MVP 4 mediates interaction with PARP4.
  • Post-translational
    modifications    Phosphorylated on Tyr residues after EGF stimulation.
    Dephosphorylated by PTPN11.
  • Cellular localizationCytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment.

    • Target information above from: UniProt accession Q14764 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      LRP antibodyLung resistance related protein antibodyLung resistance-related protein antibody
      Major vault protein antibodyMVP antibodyMVP_HUMAN antibodyVAULT 1 antibodyVAULT1 antibody
    see all

Anti-MVP antibody images

  • Western blot - MVP antibody (ab97311)
    Western blot - MVP antibody (ab97311)
    Anti-MVP antibody (ab97311) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size : 99 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MVP antibody (ab97311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MVP antibody (ab97311)
    Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ab97311 at 1/500 dilution.

References for Anti-MVP antibody (ab97311)

This product has been referenced in:
  • O'Dwyer D  et al. The proteomics of colorectal cancer: identification of a protein signature associated with prognosis. PLoS One 6:e27718 (2011). IHC-Fr ; Human . Read more (PubMed: 22125622) »

See 1 Publication for this product

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Western blot abreview for Anti-MVP antibody

 Average
Abreviews
Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal Muscle)
Loading amount 30 µg
Specification Skeletal Muscle
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

Submitted Jun 30 2012

I would like to test the Anti-MVP antibody (ab97311) for WB and IHC on mouse muscle tissues.

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I am very pleased to hear you would like to accept our offer and test ab97311 in mouse tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem th...

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