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Overview

  • Product nameMYO10 protein (Tagged-His Tag)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 1595-2058 (464 amino acids) of Human MYO10 with N terminal His tag; MWt 56kDa (UniProt Q9HD67).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 162 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesTGHDLRPLRDELYCQLIKQTNKVPHPGSVGNLYSWQILTC LSCTFLPSR GILKYLKFHLKRIREQFPGSEMEKYALFT YESLKKTKCREFVPSRDEIE ALIHRQEMTSTVYCHGGG SCKITINSHTTAGEVVEKLIRGLAMEDSRNM FALFEYN GHVDKAIESRTVVADVLAKFEKLAATSEVGDLPWKFYFKL YC FLDTDNVPKDSVEFAFMFEQAHEAVIHGHHPAPEEN LQVLAALRLQYLQ GDYTLHAAIPPLEEVYSLQRLKARI SQSTKTFTPCERLEKRRTSFLEGT LRRSFRTGSVVRQK VEEEQMLDMWIKEEVSSARASIIDKWRKFQGMNQE QAM AKYMALIKEWPGYGSTLFDVECKEGGFPQELWLGVSADAV SVYKRG EGRPLEVFQYEHILSFGAPLANTYKIVVDERE LLFETSEVVDVAKLMKA YISMIVKKRYSTTRSASSQGS SR
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91937 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        AW048724D15Ertd600eFLJ10639
        FLJ21066FLJ22268FLJ43256KIAA0799MGC131988mKIAA0799MYO10MYO10_HUMANMyosin XMyosin-XOTTMUSP00000022066Unconventional myosin 10Unconventional myosin-10
      see all
  • FunctionMyosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments (By similarity). Plays a role in regions of dynamic actin.
  • Sequence similaritiesContains 1 FERM domain.
    Contains 3 IQ domains.
    Contains 1 myosin head-like domain.
    Contains 1 MyTH4 domain.
    Contains 2 PH domains.

    • Target information above from: UniProt accession Q9HD67 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    MYO10 protein (Tagged-His Tag) images

    • SDS-PAGE - MYO10 protein (Tagged-His Tag) (ab91937)
      SDS-PAGE - MYO10 protein (Tagged-His Tag) (ab91937)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for MYO10 protein (Tagged-His Tag) (ab91937)

    ab91937 has not yet been referenced specifically in any publications.

    Publishing research using ab91937 ? Please let us know so that we can cite the reference in this datasheet

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