Anti-MYRIP antibody (ab10149)
Key features and details
- Goat polyclonal to MYRIP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MYRIP antibody -
Description
Goat polyclonal to MYRIP -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide: KDLMEPALESAVMY, corresponding to C terminal amino acids 846-859 of Human MYRIP.
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General notes
A molecular complex composed of Rab27A, MYRIP and myosin VIIa bridges retinal melanosomes to the actin cytoskeleton and thereby mediates the local trafficking of these organelles. The defect of this molecular complex is likely to account for the perinuclear mislocalization of the melanosomes observed in the retinal pigment epithelium cells of myosin VIIa-defective mice.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody notes
A molecular complex composed of Rab27A, MYRIP and myosin VIIa bridges retinal melanosomes to the actin cytoskeleton and thereby mediates the local trafficking of these organelles. The defect of this molecular complex is likely to account for the perinuclear mislocalization of the melanosomes observed in the retinal pigment epithelium cells of myosin VIIa-defective mice. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10149 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 96 kDa.
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 96 kDa. 1 hour primary incubation is recommended for this product. |
Target
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Function
Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor proteins MYO5A and MYO7A. May link RAB27A-containing vesicles to actin filaments. -
Tissue specificity
Detected in brain, skin, heart, adrenal medulla, pancreas, intestine, liver, kidney, muscle and testis. -
Sequence similarities
Contains 1 FYVE-type zinc finger.
Contains 1 RabBD (Rab-binding) domain. -
Cellular localization
Cytoplasm. In pre- and post-synaptic areas in photoreceptor cells and in the basal microvilli of retinal pigment epithelium cells. Associated with melanosomes. Colocalizes with actin filaments. - Information by UniProt
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Database links
- Entrez Gene: 25924 Human
- Omim: 611790 Human
- SwissProt: Q8NFW9 Human
- Unigene: 594535 Human
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Alternative names
- DKFZp586F1018 antibody
- Exophilin 8 antibody
- Exophilin antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab10149 has been referenced in 3 publications.
- Conte IL et al. Interaction between MyRIP and the actin cytoskeleton regulates Weibel-Palade body trafficking and exocytosis. J Cell Sci 129:592-603 (2016). PubMed: 26675235
- Song J et al. TLR4-mediated expulsion of bacteria from infected bladder epithelial cells. Proc Natl Acad Sci U S A 106:14966-71 (2009). IP ; Human . PubMed: 19706440
- Nightingale TD et al. Rab27a and MyRIP regulate the amount and multimeric state of VWF released from endothelial cells. Blood 113:5010-8 (2009). ICC/IF ; Human . PubMed: 19270261