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Overview

  • Product nameAnti-Macro H2A.2 antibodySee all Macro H2A.2 primary antibodies ...
  • Description
    Rabbit polyclonal to Macro H2A.2
  • SpecificityThe problem has been that macroH2A2 is not very ubiquitous compared to macro H2A.1. While this makes it good for commercialization purposes it is hard to prepare chromatin fractions that are enriched in this fraction.
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Macro H2A.2.

    Read Abcam's proprietary immunogen policy

    (Peptide available as ab15023.)

  • Positive controlThis antibody gave a positive signal in the following cell lysates: HeLa nuclear extract.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab4173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 40 kDa). Can be blocked with Macro H2A.2 peptide (ab15023).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionVariant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.
    • Sequence similaritiesContains 1 histone H2A domain.
      Contains 1 Macro domain.
    • Cellular localizationNucleus. Chromosome. Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin.

      • Target information above from: UniProt accession Q9P0M6 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          Core histone macro H2A2.2 antibodyCore histone macro-H2A.2 antibodyCore histone macroH2A2.2 antibody
          H2A histone family member Y2 antibodyH2AFY2 antibodyH2AW_HUMAN antibodyHistone macroH2A2 antibodyMacro H2A2 antibodymacroh2a.2 antibodymacroh2a2 antibodymH2A2 antibody
        see all

      Anti-Macro H2A.2 antibody images

      • Western blot - Anti-Macro H2A.2 antibody (ab4173)
        Western blot - Anti-Macro H2A.2 antibody (ab4173)
        Lane 1 : 2 ug of chicken erythrocyte chromatin fraction stained with ponceau.
        Lane 2 : 2 ug of chicken liver chromatin fraction stained with ponceau.
        Lanes 3 - 4 : Anti-Macro H2A.2 antibody (ab4173) at 1/500 dilution

        Lane 1 : As above
        Lane 2 : As above
        Lane 3 : 2 ug of chicken erythrocyte chromatin fraction.
        Lane 4 : 2 ug of chicken liver chromatin fraction.

        Secondary
        Lanes 3 - 4 : Secondary antibody used at a dilution of 1/3000.


        Performed under reducing conditions.

        Predicted band size : 40 kDa

        Juan Ausio

      • Western blot - Macro H2A.2 antibody (ab4173)
        Western blot - Macro H2A.2 antibody (ab4173)
        All lanes : Anti-Macro H2A.2 antibody (ab4173) at 1 µg/ml

        Lane 1 : HeLa nuclear extract
        Lane 2 : HeLa nuclear extract with Macro H2A.2 peptide (ab15023) at 1 µg/ml

        Lysates/proteins at 20 µg per lane.


        Predicted band size : 40 kDa
        Observed band size : 50 kDa (why is the actual band size different from the predicted?)

      References for Anti-Macro H2A.2 antibody (ab4173)

      This product has been referenced in:
      • Boulard M  et al. Histone variant macroH2A1 deletion in mice causes female-specific steatosis. Epigenetics Chromatin 3:8 (2010). WB ; Mouse . Read more (PubMed: 20359320) »

      See 1 Publication for this product

      Publishing research using ab4173 ? Please let us know so that we can cite the reference in this datasheet

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"