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Macrophage Inflammatory Protein 1 alpha / CCL3 (Active) (ab9782)

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Overview

  • Product nameMacrophage Inflammatory Protein 1 alpha / CCL3 (Active)See all Macrophage Inflammatory Protein 1 alpha / CCL3 proteins and peptides ...
  • Protein descriptionRecombinant full length Rat MIP1 alpha protein
  • Expression hostE. coli

    • Properties

  • Purification notesSterile filtered Greater than 95% pure by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • FormLyophilised:Add 200ul of dH2O.
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Concentration information loading...
  • Additional notesThe biological activity of this product is determined by its ability to chemoattract rat peritoneal macrophages using a concentration range of 50.0-100.0 ng/ml.
  • Sequence notesMIP-1-alpha is an 7.8 kDa protein containing 69 amino acid residues: APYGADTPTA CCFSYGRQIP RKFIADYFET SSLCSQPGVI FLTKRNRQIC ADPKETWVQE YITELELNA
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab9782 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab9781.

    • Protein info

    • Alternative names
        C C motif chemokine 3CCL 3CCL3
        CCL3_HUMANChemokine C C motif ligand 3Chemokine CC Motif Ligand 3G0/G1 switch regulatory protein 19 1G0/G1 switch regulatory protein 19-1G0S19 1G0S19 1 proteinHeparin binding chemotaxis proteinL2G25BLD78 alphaLD78-alpha(4-69)LD78ALPHAMacrophage inflammatory protein 1 alphaMacrophage inflammatory protein 1-alphaMIP 1 alphaMIP 1AMIP-1-alphaMIP-1-alpha(4-69)MIP1APAT 464.1SCYA 3SCYA3SIS alphaSIS alphaSIS betaSIS-betaSmall inducible cytokine A3Small-inducible cytokine A3Tonsillar lymphocyte LD78 alpha proteinTonsillar lymphocyte LD78 alpha protein
      see all
  • FunctionMonokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications    N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P10147 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for Macrophage Inflammatory Protein 1 alpha / CCL3 (Active) (ab9782)

    ab9782 has not yet been referenced specifically in any publications.

    Publishing research using ab9782 ? Please let us know so that we can cite the reference in this datasheet

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    Dear Jo, The customerhas no specific lot (i'm not sure if he has purchased the product yet), I would be very grateful if you could send the assay information (you can send attachements to sarah.mardle@abcam.com if this is easier!). Many many thanks, ...

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    We have just been informed by the source of ab9782 that the the procedure for the chemotaxis protocol on rat peritoneal macrophages can be found in the references: – Frevert et al. Journal of Immunology 154: 335-344 -and Jones et al. Journal of Immuno...

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    Hello! I'm interested in this product and would like to know if I have understood it correctly that it is of mouse origin but the activity has been tested on rat cells. Do you know the how similar the amino acid identity is between mouse and rat MIP1 a...

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    Thank you for your recent e-mail regarding the PIP1 alpha protein ab9782. I can confirm that this product is recombinant rat MIP-1 alpha, a 7.8 kDa protein containing 69 amino acid residues including the four highly conserved cysteine residues present ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"