Purification notesab106028 is an IgG fraction antibody purified from monospecific
antiserum by a multi-step process which includes delipidation, salt
fractionation and ion exchange chromatography followed by extensive
dialysis against the buffer stated. This antibody has been heated to 56°C for 30 minutes.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Detects a band of approximately 15 kDa (predicted molecular weight: 10 kDa).
Use at an assay dependent dilution.
FunctionMonokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
Post-translational modificationsN-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes.