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Macrophage Inflammatory Protein 1 beta protein (Active) (ab88198)

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Overview

  • Product nameMacrophage Inflammatory Protein 1 beta protein (Active)See all Macrophage Inflammatory Protein 1 beta proteins and peptides ...
  • Protein descriptionRecombinant full length Pig Macrophage Inflammatory Protein 1 beta (without signal peptide), expressed in Yeast. MW 7.8kDa. (aas 24-92), 69 aas. Swiss prot Q711P4.
  • Expression hostSaccharomyces cerevisiae

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesPurification: Ion-exchange chromatography.
  • Biological activityThe biological activity of recombinant swine Macrophage Inflammatory Protein 1 beta was determined in a chemotaxis assay using a Boyden chamber with freshly isolated swine PBMC monocytes using a Percoll gradient enrichment method. Biological activity typically peaks at 10 ng/mL.
  • FormLyophilised:Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein. Stable for up to twelve months from date of receipt at -20°C. Stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 10% Trehalose, 1X PBS
  • Concentration information loading...
  • Sequence notesAPMGSDPPTS CCFTYTVRKL PRNFVTDYYE TSSLCSQPAV VFQTKKGRQV CANPSDDWVQ EYMDDLELN
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab88198 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    Functional Studies FuncS: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab136393.

    • Protein info

    • Alternative names
        MIP 1 beta Secreted protein G 26ACT 2
        ACT-2ACT2AT744.1AT744.2C C motif chemokine 4C C motif chemokine 4 likeCC chemokine ligand 4CC chemokine ligand 4L1d2CC chemokine ligand 4L2CCL4CCL4_HUMANCCL4Lccl4l 1CCL4L1Chemokine (C C motif) ligand 4Chemokine (C C motif) ligand 4 like 1Chemokine (C C motif) ligand 4 like 1, telomericChemokine (C C motif) ligand 4 like 2Chemokine CC Motif Ligand 4G 26G 26 T lymphocyte secreted proteinG-26 T-lymphocyte-secreted proteinHC21LAG 1LAG-1LAG1Lymphocyte activation gene 1Lymphocyte activation gene 1 proteinMacrophage inflammatory protein 1 betaMacrophage inflammatory protein 1-betaMacrophage inflammatory protein 1b2MGC104418MGC126025MGC126026MIP-1-betaMIP-1-beta(1-69)MIP-1-beta(3-69)MIP1 betaMIP1BMIP1B1Monocyte adherence induced protein 5 alphaPAT 744Protein H400SCYA2SCYA4SCYA4LSecreted protein G26SIS gammaSIS-gammaSmall inducible cytokine A4Small inducible cytokine A4 (homologous to mouse Mip 1b)Small-inducible cytokine A4T cell activation protein 2T-cell activation protein 2
      see all
  • FunctionMonokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications    N-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P13236 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for Macrophage Inflammatory Protein 1 beta protein (Active) (ab88198)

    ab88198 has not yet been referenced specifically in any publications.

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