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Macrophage Inflammatory Protein 3 alpha Human ELISA Kit (ab100599)

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Overview

  • Product nameMacrophage Inflammatory Protein 3 alpha Human ELISA KitSee all Macrophage Inflammatory Protein 3 alpha kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 1.5 pg/ml
  • Range
    0.8 pg/ml - 600 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.25 83% - 102%
    Serum 95.39 83% - 103%
    Plasma 93.88 82% - 102%

  • Species reactivity
    Reacts with: Human
  • Product overview

    Macrophage Inflammatory Protein 3 alpha is a CC chemokine with a selective chemotactic activity for lymphocytes and dendritic cells. Macrophage Inflammatory Protein 3 alpha is expressed predominantly in lymph nodes, appendix, peripheral blood leukocytes, fetal liver, fetal lung and several cell lines. It plays an important role in the regulation of DC trafficking, and recruitment and activation of T cells.

     

    ab100599 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Macrophage Inflammatory Protein 3 alpha in serum, plasma and cell culture supernatants.

     

    This assay employs an antibody specific for human Macrophage Inflammatory Protein 3 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and Macrophage Inflammatory Protein 3 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Macrophage Inflammatory Protein 3 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Macrophage Inflammatory Protein 3 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

     

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • FunctionChemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213.
  • Tissue specificityExpressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications    C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P78556 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        Beta chemokine exodus 1Beta-chemokine exodus-1C C motif chemokine 20
        CC chemokine LARCCcl20CCL20(2-70)CCL20_HUMANChemokine (C C motif) ligand 20CKb4Exodus 1LARCLiver and activation regulated chemokineLiver and activation-regulated chemokineMacrophage inflammatory protein 3 alphaMIP 3 alphaMIP 3aMIP-3-alphaMIP3 alphaMIP3AMIP3alphaSCYA20Small inducible cytokine A20Small inducible cytokine subfamily A (Cys Cys) member 20Small-inducible cytokine A20ST38
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100599 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Macrophage Inflammatory Protein 3 alpha Human ELISA Kit images

      • ELISA - Macrophage Inflammatory Protein 3 alpha Human ELISA Kit (ab100599)
        ELISA - Macrophage Inflammatory Protein 3 alpha Human ELISA Kit (ab100599)

      Protocols

      References for Macrophage Inflammatory Protein 3 alpha Human ELISA Kit (ab100599)

      ab100599 has not yet been referenced specifically in any publications.

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