Anti-Macrophage Inflammatory Protein 3 alpha antibody (ab9829)
Overview
- Product nameAnti-Macrophage Inflammatory Protein 3 alpha antibodySee all Macrophage Inflammatory Protein 3 alpha primary antibodies ...
- DescriptionRabbit polyclonal to Macrophage Inflammatory Protein 3 alpha
- Tested applicationsIHC-FoFr, IHC-P, WB, ELISA, Neutralising, ICC/IF more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Highly pure (>98%) recombinant hMIP-3-alpha (human macrophage inflammatory protein-3 alpha)
Properties
- FormLyophilised:Reconstitute with 200µl of sterile water. The reconstituted antibody is stable for at least 2 weeks at 2-8C and at least 6 months at -20C
- Storage instructionsShipped at 4°C. Store at -20ºC.
- Storage bufferPBS, pH 7.4, no preservative, sterile filtered
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Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- Isotypeunknown
- Light chain typeunknown
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab9829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-FoFr | IHC-FoFr: Use at an assay dependent dilution. PubMed: 19305396 |
| IHC-P | IHC-P: Use a concentration of 15 µg/ml. |
| WB | WB: Use at an assay dependent concentration. To detect hMIP-3-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-3-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| ELISA | ELISA: Use at an assay dependent concentration. Can be paired for ELISA with Mouse monoclonal to Macrophage Inflammatory Protein 3 alpha (ab9349). To detect hMIP-3-alpha by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-3-alpha. |
| Neutralising | Neut: Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-3-alpha (100 ng/ml), a concentration of 15.0 - 18.0 µg/ml of this antibody is required. |
| ICC/IF | ICC/IF: Use at an assay dependent dilution. PubMed: 20952674 |
Target
- FunctionChemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213.
- Tissue specificityExpressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon.
- Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
- Post-translational
modificationsC-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes. - Cellular localizationSecreted.
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Database links
- Entrez Gene: 6364 Human
- Entrez Gene: 20297 Mouse
- Entrez Gene: 29538 Rat
- Omim: 601960 Human
- SwissProt: P78556 Human
- SwissProt: O89093 Mouse
- SwissProt: P97884 Rat
- Unigene: 75498 Human
- Unigene: 116739 Mouse
- Unigene: 10722 Rat
see all
Target information above from: UniProt accession
P78556
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Beta-chemokine exodus-1 antibodyC-C motif chemokine 20 antibodyCC chemokine LARC antibody
- CCL20 antibodyCCL20(2-70) antibodyCCL20_HUMAN antibodyChemokine (C C motif) ligand 20 antibodyChemokine CC motif ligand 20 antibodyCKb4 antibodyExodus 1 antibodyLARC antibodyLiver and activation-regulated chemokine antibodyMacrophage inflammatory protein 3 alpha antibodyMIP 3 alpha antibodyMIP 3A antibodyMIP-3-alpha antibodyMIP3A antibodySCYA20 antibodySmall inducible cytokine A20 antibodySmall inducible cytokine subfamily A (Cys Cys) member 20 antibodySmall-inducible cytokine A20 antibodyST38 antibody
see all
Anti-Macrophage Inflammatory Protein 3 alpha antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Macrophage Inflammatory Protein 3 alpha antibody (ab9829)ab9829 (1µg/ml) staining Macrophage Inflammatory Protein 3 alpha in human tonsil (left panel) using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of vesicles.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Macrophage Inflammatory Protein 3 alpha antibody (ab9829)Image from Tjomsland V et al., Clin Dev Immunol. 2011;2011:212810. Epub 2011 Dec 6. Fig 3.; doi:10.1155/2011/212810; Clinical and Developmental Immunology, Volume 2011 (2011), Article ID 212810Immunohistochemical analysis of pancreatic ductal adenocarcinoma tissue, staining Macrophage Inflammatory Protein 3 alpha with ab9829.
Antigen retrieval was performed by heat mediation in citrate buffer (pH 6.0). Tissue was blocked with 1% BSA for 10 min. The samples incubated overnight with primary antibody (1/40). Staining was detected using DAB. Sections were counterstained with hematoxylin.
References for Anti-Macrophage Inflammatory Protein 3 alpha antibody (ab9829)
This product has been referenced in:
- Tjomsland V et al. The desmoplastic stroma plays an essential role in the accumulation and modulation of infiltrated immune cells in pancreatic adenocarcinoma. Clin Dev Immunol 2011:212810 (2011). IHC-P ; Human . Read more (PubMed: 22190968) »
- Turner JE et al. CCR6 recruits regulatory T cells and Th17 cells to the kidney in glomerulonephritis. J Am Soc Nephrol 21:974-85 (2010). Read more (PubMed: 20299360) »
