Anti-Macrophage Inflammatory Protein 3 alpha antibody (ab9829)

Overview

  • Product nameAnti-Macrophage Inflammatory Protein 3 alpha antibodySee all Macrophage Inflammatory Protein 3 alpha primary antibodies ...
  • Description
    Rabbit polyclonal to Macrophage Inflammatory Protein 3 alpha
  • Tested applicationsIHC-FoFr, IHC-P, WB, ELISA, Neutralising, ICC/IF, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Highly pure (>98%) recombinant hMIP-3-alpha (human macrophage inflammatory protein-3 alpha)

Properties

  • FormLyophilised:Reconstitute with 200µl of sterile water. The reconstituted antibody is stable for at least 2 weeks at 2-8C and at least 6 months at -20C
  • Storage instructionsShipped at 4°C. Store at -20ºC.
  • Storage bufferPBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • Isotypeunknown
  • Light chain typeunknown
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab9829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 19305396
IHC-P Use a concentration of 15 µg/ml.
WB Use at an assay dependent concentration. To detect hMIP-3-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-3-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. Can be paired for ELISA with Mouse monoclonal to Macrophage Inflammatory Protein 3 alpha (ab9349). To detect hMIP-3-alpha by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-3-alpha.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-3-alpha (100 ng/ml), a concentration of 15.0 - 18.0 µg/ml of this antibody is required.
ICC/IF Use at an assay dependent concentration. PubMed: 20952674
ICC Use at an assay dependent concentration. PubMed: 24633013

Target

  • FunctionChemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213.
  • Tissue specificityExpressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta-chemokine exodus-1 antibody
    • C-C motif chemokine 20 antibody
    • CC chemokine LARC antibody
    • CCL20 antibody
    • CCL20(2-70) antibody
    • CCL20_HUMAN antibody
    • Chemokine (C C motif) ligand 20 antibody
    • Chemokine CC motif ligand 20 antibody
    • CKb4 antibody
    • Exodus 1 antibody
    • LARC antibody
    • Liver and activation-regulated chemokine antibody
    • Macrophage inflammatory protein 3 alpha antibody
    • MIP 3 alpha antibody
    • MIP 3A antibody
    • MIP-3-alpha antibody
    • MIP-3a antibody
    • MIP3A antibody
    • SCYA20 antibody
    • Small inducible cytokine A20 antibody
    • Small inducible cytokine subfamily A (Cys Cys) member 20 antibody
    • Small-inducible cytokine A20 antibody
    • ST38 antibody
    see all

Anti-Macrophage Inflammatory Protein 3 alpha antibody images

  • ab9829 (1µg/ml) staining Macrophage Inflammatory Protein 3 alpha in human tonsil (left panel) using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of vesicles.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

  • Immunohistochemical analysis of pancreatic ductal adenocarcinoma tissue, staining Macrophage Inflammatory Protein 3 alpha with ab9829.

    Antigen retrieval was performed by heat mediation in citrate buffer (pH 6.0). Tissue was blocked with 1% BSA for 10 min. The samples incubated overnight with primary antibody (1/40). Staining was detected using DAB. Sections were counterstained with hematoxylin.

References for Anti-Macrophage Inflammatory Protein 3 alpha antibody (ab9829)

This product has been referenced in:
  • Wu HX  et al. Decidual stromal cells recruit Th17 cells into decidua to promote proliferation and invasion of human trophoblast cells by secreting IL-17. Cell Mol Immunol 11:253-62 (2014). ICC ; Human . Read more (PubMed: 24633013) »
  • Shuman Moss LA  et al. Dietary intake of a plant phospholipid/lipid conjugate reduces lung cancer growth and tumor angiogenesis. Carcinogenesis N/A:N/A (2014). Read more (PubMed: 24510111) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step soution from Dako as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate
Sample Human Tissue sections (tonsil)
Specification tonsil
Permeabilization No
Fixative Formaldehyde
Username

Dr. R Berahovich

Verified customer

Submitted Aug 06 2013

This Rabbit polyclonal is a purified antibody with a IgG concentration of 0.5MG/ML. Humans, mice, and rats have multiple IgG subclasses, whereas rabbits have only a single class of IgG. As this is a polyclonal the product is result of many differe...

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Gracias por contactarnos.

Para testar este anticuerpo en Western Blot usamos la proteína recombinante MIP-3 alfa diluida. Respecto al protocolo usado, se usó una concentración de anticuerpo de 0.1-0.2 ug/ml, y condiciones...

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Thank you for contacting us.

According to the Unitprot entry for this proteinhttp://www.uniprot.org/uniprot/O89093, CCL20 is prominently expressed in the small intestine, colon and appendix. It is also found in thymus, spleen, lymph node a...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Lymph Node)
Specification Lymph Node
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Jul 03 2012

DISCOUNT CODE: ***
Expiration date: *********

I am very pleased to hear you would like to accept our offer and test ab9829 inrat tissue. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, ...

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email bien reçu. J'ai mis en place l'envoi de l'anticorps de remplacement et je vous ai communiqué le numéro de cette commande gratuite. Avez-vous bien reçu cette confirmation de votre coté?

Merci de nous avoir contactés.

Comme convenu j'ai mis en place l'envoi d'une unité gratuitede ab85032 en remplacement du ab9829 qui n'a pas fonctionné comme attendu en IHC-P. Le numéro de commande de remplacement ...

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as a replacement we can offer :
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Thank you very much for checking with the customer.

I am very sorry to hear that the suggestions did not help to improve the results. I must conclude that the antibody vial has been faulty. I am very sorry for this inconvenience and would lik...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"