Macrophage Inflammatory Protein 3 beta antibody (ab10389)

Product Name 

Macrophage Inflammatory Protein 3 beta antibody

Product type 

Primary antibodies

Description 

Goat polyclonal to Macrophage Inflammatory Protein 3 beta

Immunogen 

Purified recombinant mouse MIP-3beta expressed in E. coli.

Reacts with 
(species key)

Hu, Ms

Specificity 

By Immunoblotting and ELISA the antibody shows approximately 5% cross-reactivity with recombinant human MIP3 beta.
In addition, the antibody shows no crossreactivity with other chemokines tested:
rh6Ckine, rm6Ckine, rhBLC/BCA 1, rmC10, rrCINC1, rrCINC2 alpha, rrCINC2 beta, rmCRG2, rhENA78, rhEotaxin, rmEotaxin, rhEotaxin 2, rhFractalkine, rmFractalkine, rhGCP2, rmGCP2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhHCC-1, rhI309, rhHCC4, rhIL8, rhIP10, rhI-TAC, rmJE, rhLeukotactin-1, rmLymphotactin, rmMARC, rhMCP1, rhMCP2, rhMCP3, rhMCP4, rmMCP5, rhMDC, rmMDC, rhMIG, rmMIG, rvMIPI, rhMIP1 alpha, rmMIP1 alpha, rhMIP1 beta (ACT II), rvMIPII, rvMIPIII, rmMIP1 beta, rhMIP1 delta, rmMIP1 gamma, rmMIP2, rhMIP3 alpha, rrMIP3 alpha, rhMIP3 beta, rmMIP3 beta, rhMPIF1, rhNAP2, rhPARC, rhRANTES, rmRANTES, rhSDF1 alpha, rmSDF1 alpha, rhSDF1 beta, rhTarc, rmTCA3, rhTeck, rmTeck.

Tested applications 
(see key)

ELISA, IHC-P, Neut, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: Use at a concentration of 0.5 - 1 µg/ml to detect a limit of 0.16 ng/well of recombinant mouse MIP3 beta..
IHC-P: Use at an assay dependent dilution (PMID 19917684).
Neut: Use at a concentration of 1 - 3 µg/ml to neutralize 50% of the bioactivity due to 0.1 ug/ml recombinant mouse MIP3 beta using cultured lymphocytes.
WB: Use at a concentration of 0.1 - 0.2 µg/ml using mouse MIP3 beta at 1 ng/lane under non-reducing and reducing conditions. Predicted molecular weight: 11 kDa.

To measure the ability of the antibody to neutralize the bioactivity of mouse MIP3 beta, recombinant mouse MIP3 beta was incubated with various concentrations of the antibody for 30 minutes in a microwell plate. Following preincubation, 35 ul of cytokine-antibody solution (containing antibody at concentrations of 0.01- 200 ug/ml and recombinant mouse MIP3 beta at 0.1 ug/ml) was transferred to the lower compartment of a 96-well chemotaxis chamber. The chamber was assembled using a PVP-free polycarbonate filter and 2 x 10⁶ cells/well were added to the top chamber. After the plate was incubated at 37°C for 3 hours in a humidified CO₂ incubator, the cells that migrated through to the lower chamber were stained using MTT and the optical density was measured. The ND50 is the concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when the cytokine is present at a concentration just high enough to elicit a maximum response.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Immunology >> Innate Immunity >> Chemokines >> Beta Chemokines (CC)
Immunology >> Innate Immunity >> Macrophage / Inflamm.

Relevance 

MIP3 chemokines are among many novel beta chemokines recently identified through bioinformatics in the Expressed Sequence Tag (EST) database. They are distantly related to other beta chemokines (20-30% aa sequence homology). MIP3 chemokines are useful probes for studying the biology of inflammation and cell migration. Their expression is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL10. MIP3 beta is also known as ELC (EBI1-1 ligand chemokine) and is constitutively expressed in various lymphoid tissues (thymus, lymph nodes, appendix and spleen). MIP3 beta is a chemoattractant for cultured human lymphocytes, dendritic cells, human T lymphoblastoid CEM NKR2 and hemopoietic progenitor cells. It may be involved in G Protein activation in human NK cells. MIP3 beta is a unique functional ligand for CCR7. The MIP3 beta gene has been mapped to chormosome 9p13.

Database links 

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: PBS

Purification notes 

The antibody is purified using mouse MIP3 beta affinity chromatography.

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Endotoxin level is < 10 ng per mg antibody as determined by the LAL method.

References for Macrophage Inflammatory Protein 3 beta antibody (ab10389)

  This product has been used in:

Shao Z et al. Fms-Like Tyrosine Kinase 3 Ligand Regulates Migratory Pattern and Antigen Uptake of Lung Dendritic Cell Subsets in a Murine Model of Allergic Airway Inflammation. J Immunol 183:7531-7538 (2009). IHC-P; Mouse. PubMed: 19917684

If you publish research using ab10389 please let us know so that we can cite the reference on this datasheet.

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