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Products:Immunology >> Innate Immunity >> Macrophage / Inflamm.
Overview
Product name
Anti-Macrophage Inflammatory Protein 3 beta antibody
See all Macrophage Inflammatory Protein 3 beta products (5) ...
Description
Rabbit polyclonal to Macrophage Inflammatory Protein 3 beta
Tested applications
WB, ELISA, Neutralisingmore details
Cross reactivity
Reacts with
Human
Immunogen
Highly pure (>98%) recombinant hMIP-3-b (human macrophage inflammatory protein-3 beta)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, pH 7.4, no preservative, sterile filtered
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
unknown
Light chain type
unknown
Research areas
Immunology >> Innate Immunity >> Chemokines >> Beta Chemokines (CC)
Immunology >> Innate Immunity >> Macrophage / Inflamm.
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration. (To detect hMIP-3-b by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-3-b is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.)
ELISA: Use at an assay dependent concentration. Can be paired for ELISA with Mouse monoclonal to Macrophage Inflammatory Protein 3 beta (ab9351). (To detect hMIP-3-b by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-3-b.)
Neut: Use at an assay dependent concentration. (To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-3-b (100 ng/ml), a concentration of 2.7 - 4.0 µg/ml of this antibody is required.)
Target
Relevance
MIP3 chemokines are among many novel beta chemokines recently identified through bioinformatics in the Expressed Sequence Tag (EST) database. They are distantly related to other beta chemokines (20-30% aa sequence homology). MIP3 chemokines are useful probes for studying the biology of inflammation and cell migration. Their expression is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL10. MIP3 beta is also known as ELC (EBI1-1 ligand chemokine) and is constitutively expressed in various lymphoid tissues (thymus, lymph nodes, appendix and spleen). MIP3 beta is a chemoattractant for cultured human lymphocytes, dendritic cells, human T lymphoblastoid CEM NKR2 and hemopoietic progenitor cells. It may be involved in G Protein activation in human NK cells. MIP3 beta is a unique functional ligand for CCR7. The MIP3 beta gene has been mapped to chormosome 9p13.
Cellular localization
Secreted
Alternative names
- Beta chemokine exodus 3 antibody
- C C chemokine ligand 19 antibody
- CC chemokine ligand 19 antibody
see all
Database links
- Entrez Gene: 6363 Human
- Omim: 602227 Human
- SwissProt: Q5VZ75 Human
- SwissProt: Q6IBD6 Human
- SwissProt: Q99731 Human
- Unigene: 50002 Human
References for Anti-Macrophage Inflammatory Protein 3 beta antibody (ab9831)
ab9831 has not yet been referenced specifically in any publications.
Publishing research using ab9831? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"