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Product Name
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Macrophage Inflammatory Protein 3 beta protein
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Product type
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Proteins and Peptides
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Peptide
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Highly pure (>98%) recombinant human Macrophage Inflammatory Protein 3 beta.
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Tested applications
(see key)
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FuncS, SDS-PAGE
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Application notes
(see key)
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Recommended dilutions
FuncS: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
This peptide can be used with studies using ab9351 and ab9831.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Immunology >> Innate Immunity >> Chemokines >> Beta Chemokines (CC)
Immunology >> Innate Immunity >> Macrophage / Inflamm.
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Relevance
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MIP3 chemokines are among many novel beta chemokines recently identified through bioinformatics in the Expressed Sequence Tag (EST) database. They are distantly related to other beta chemokines (20-30% aa sequence homology). MIP3 chemokines are useful probes for studying the biology of inflammation and cell migration. Their expression is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL10. MIP3 beta is also known as ELC (EBI1-1 ligand chemokine) and is constitutively expressed in various lymphoid tissues (thymus, lymph nodes, appendix and spleen). MIP3 beta is a chemoattractant for cultured human lymphocytes, dendritic cells, human T lymphoblastoid CEM NKR2 and hemopoietic progenitor cells. It may be involved in G Protein activation in human NK cells. MIP3 beta is a unique functional ligand for CCR7. The MIP3 beta gene has been mapped to chormosome 9p13.
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Storage buffer
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n/a
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Purification notes
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Sterile filtered
Greater than 99% pure by SDS-PAGE and HPLC analyses.
Endotoxin level is less than 0.1 ng per µg (1EU/µg).
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Form
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Lyophilised
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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The biological activity of this product was determined by its ability to chemoattract human T cells using a concentration of 10.0-50.0 ng/ml.
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Expression system
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E. coli
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Sequence
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Human MIP-3-beta is an 8.8 kDa protein containing 77 amino acid residues:
GTNDAEDCCL SVTQKPIPGY IVRNFHYLLI KDGCRVPAVV FTTLRGRQLC APPDQPWVER IIQRLQRTSA KMKRRSS
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Macrophage Inflammatory Protein 3 beta protein is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to Macrophage Inflammatory Protein 3 beta
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