Macrophage Stimulating Protein Human ELISA Kit (ab100612)
- Product nameMacrophage Stimulating Protein Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 8 pg/ml
- Range8.23 pg/ml - 2000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 97.25 86% - 106% Serum 94.48 83% - 104% Plasma 95.29 82% - 105%
- Species reactivityReacts with: Human
- Product overview
ab100612 Macrophage Stimulating Protein (MSP) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Macrophage Stimulating Protein coated on a 96-well plate. Standards and samples are pipetted into the wells and Macrophage Stimulating Protein present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Macrophage Stimulating Protein antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Macrophage Stimulating Protein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 200X HRP-Streptavidin Concentrate Item G 1 x 200µl 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent A animal serum . For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml Detection Antibody MSP: biotinylated anti-human MSP 2 vials MSP Microplate (12 strips x 8 wells) coated with anti-human MSP 1 unit MSP Standard Human(recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
- DNF15S2DNF15S2hHepatocyte growth factor like proteinHepatocyte growth factor like protein homologHGFLMacrophage stimulating 1 Macrophage stimulatory proteinMSPMST1NF15S2
Our Abpromise guarantee covers the use of ab100612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Macrophage Stimulating Protein Human ELISA Kit (ab100612)
ab100612 has not yet been referenced specifically in any publications.