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Macrophage Stimulating Protein Human ELISA Kit (ab100612)

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Overview

  • Product nameMacrophage Stimulating Protein Human ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 8 pg/ml
  • Range
    8.23 pg/ml - 2000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 97.25 86% - 106%
    Serum 94.48 83% - 104%
    Plasma 95.29 82% - 105%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab100612 Macrophage Stimulating Protein (MSP) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human Macrophage Stimulating Protein coated on a 96-well plate. Standards and samples are pipetted into the wells and Macrophage Stimulating Protein present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Macrophage Stimulating Protein antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Macrophage Stimulating Protein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • RelevanceMacrophage Stimulating Protein contains four kringle domains and a serine protease domain, similar to that found in hepatic growth factor. Despite the presence of the serine protease domain, the encoded protein may not have any proteolytic activity. The receptor for this protein is RON tyrosine kinase, which upon activation stimulates ciliary motility of ciliated epithelial lung cells. This protein is secreted and cleaved to form an alpha chain and a beta chain bridged by disulfide bonds.
    • Alternative names
        D3F15S2D3F15S2hD9H3F15S2
        DNF15S2DNF15S2hHepatocyte growth factor like proteinHepatocyte growth factor like protein homologHGFLMacrophage stimulating 1 Macrophage stimulatory proteinMSPMST1NF15S2
      see all

    Applications

    Our Abpromise guarantee covers the use of ab100612 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    ELISA ELISA

    Macrophage Stimulating Protein Human ELISA Kit images

    • ELISA - Macrophage Stimulating Protein Human ELISA Kit (ab100612)
      ELISA - Macrophage Stimulating Protein Human ELISA Kit (ab100612)

    Protocols

    References for Macrophage Stimulating Protein Human ELISA Kit (ab100612)

    ab100612 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"