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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Overview
Product name
Anti-Mad antibody
See all Mad products (2) ...
Description
Goat polyclonal to Mad
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: RIKLQDSHKACLGL, corresponding to C terminal amino acids 208-221 of Human Mad.
RIKLQDSHKA CLGL
Positive control
U937 cell lysate.
General notes
Principal Names - MAD: MAX dimerization protein;
GenBank Accession Number – NP_002348
LocusLink ID - 4084
Official Gene Symbol - MAD
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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Western blot - Mad antibody (ab9699)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 25 kDa in U937 cell lysates (predicted molecular weight: 25 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transcriptional repressor. MAD binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MAD thus antagonizes MYC transcriptional activity by competing for MAX.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ05195
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MAD antibody
- MAD protein antibody
- MAD1 antibody
see all
Database links
- Entrez Gene: 4084 Human
- SwissProt: Q05195 Human
- Unigene: 468908 Human
Anti-Mad antibody images:
Western blot - Mad antibody (ab9699)
Predicted band size : 25 kDa
ab9699 (1µg/ml) staining of U937 lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.
References for Anti-Mad antibody (ab9699)
ab9699 has not yet been referenced specifically in any publications.
Publishing research using ab9699? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"