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Products:Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Anti-Mad2L1 antibody [17D10] (ab10691)
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab10691 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
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What is the epitope recognized by this antibody? |
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ANSWER: |
The epitope recognized by this antibody has not been mapped. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - Anti-Mad2L1 antibody [17D10] (ab10691)](/ps/datasheet/Images/10/ab10691/ab10691_1.jpg)
Western blot - Anti-Mad2L1 antibody [17D10] (ab10691)
Predicted band size : 24.6 kDa
Western blot using ab10691 at 1/500.
Lane 1: Hela Nuclear
Lane 2: Hela
Lane 3: A431
Lane 4: Jurkat
Lane 5: 293
Mad2L1 predicted size: 24.6kD.
Secondary ab: Rabbit polyclonal to Mouse IgG H&L (HRP) ab6728 (1/5000)
Exposure time: 1min
20µg of lysate per lane.
![Western blot - Mad2L1 antibody [17D10] (ab10691)](/ps/datasheet/images/10/ab10691/Mad2L1-Primary-antibodies-ab10691-3.jpg)
Western blot - Mad2L1 antibody [17D10] (ab10691)
Predicted band size : 24.6 kDa
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
Mad2L1 antibody (ab10691) at 1/1000 (in TBS for 90 minutes at 22°C) + whole tissue lysate of Human liver biopsy (chronic hepatitis C - (HepC) or non-viral (NVH)) patients at 50µg.
Secondary Antibody: An HRP-conjugated Goat anti-mouse polyclonal (1/100000).
Blocking Step: 5% Milk for 16 hours at 4°C
This image is courtesy of an Abreview submitted by Saira Sarfraz
![Mad2L1 antibody [17D10] for Western blot in Human (10691)](/ps/datasheet/images/10/ab10691/Mad2L1-Primary-antibodies-ab10691-2.jpg)
Mad2L1 antibody [17D10] for Western blot in Human (10691)
![Mad2L1 antibody [17D10] for ICC/IF in Human (10691)](/ps/Reviews/Images/ab6414_23873.jpg)
Mad2L1 antibody [17D10] for ICC/IF in Human (10691)
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