Anti-Mad2L1 antibody [4D2] (ab114036)

Overview

• Product nameAnti-Mad2L1 antibody [4D2]See all Mad2L1 primary antibodies ...
• Description
  Mouse monoclonal [4D2] to Mad2L1
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant full length Human Mad2L1 produced in HEK293T cells (NP_002349).

• Positive controlHEK293T cell lysate transfected with or pCMV6-ENTRY Mad2L1; COS7 cells transiently transfected by pCMV6-ENTRY Mad2L1.
• General notesStable for 12 months from date of receipt.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.30
  Preservative: 0.02% Sodium azide
  Constituents: 48% PBS, 50% Glycerol, 1% BSA
• Concentration information loading...
• PurityProtein A purified
• Purification notesab114036 is purified from Mouse ascites fluids by affinity chromatography.
• Clonality Monoclonal
• Clone number4D2
• IsotypeIgG2a
• Research Areas
  • Cancer
  • Cell cycle
  • Cell division

  • Tags & Cell Markers
  • Subcellular Markers
  • Nucleus
  • Other Nuclear Bodies

  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Other cell division antibodies

  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Spindle

Applications

Target

• FunctionComponent of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
• Sequence similaritiesBelongs to the MAD2 family.
  Contains 1 HORMA domain.
• DomainThe protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. The protein in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and of MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed form, and thereby promotes interaction with CDC20.
• Post-translational
  modificationsPhosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20.
• Cellular localizationNucleus. Chromosome > centromere > kinetochore. Cytoplasm. Recruited by MAD1L1 to unattached kinetochores (Probable). Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD.

Target information above from: UniProt accession Q13257 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4085 Human
  • Omim: 601467 Human
  • SwissProt: Q13257 Human
  • Unigene: 591697 Human

• Alternative names
  HsMAD 2 antibodyHsMAD2 antibodyMAD 2 antibody

  MAD 2A antibodyMAD2 like 1 antibodyMAD2 like protein 1 antibodyMAD2 mitotic arrest deficient like 1 antibodyMAD2-like protein 1 antibodyMad2l1 antibodyMD2L1_HUMAN antibodyMitotic arrest deficient 2-like protein 1 antibodyMitotic arrest deficient yeast homolog like 1 antibodyMitotic spindle assembly checkpoint protein MAD2A antibody

  see all

Anti-Mad2L1 antibody [4D2] images

• 

  Western blot - Anti-Mad2L1 antibody [4D2] (ab114036)
  All lanes : Anti-Mad2L1 antibody [4D2] (ab114036) at 1/1000 dilution
  
  Lane 1 : HEK293T cell lysate transfected with the pCMV6-ENTRY control cDNA
  Lane 2 : HEK293T cell lysate transfected with the pCMV6-ENTRY Mad2L1 cDNA
  
  Lysates/proteins at 5 µg per lane.
  
  
  Predicted band size : 24 kDa
  
• 

  Flow Cytometry - Anti-Mad2L1 antibody [4D2] (ab114036)
  Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY Mad2L1, using ab114036 at 1/100.