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Anti-Mad2L1 antibody
See all Mad2L1 products (8) ...
Rabbit polyclonal to Mad2L1
WB, IFmore details
Reacts with
Human
Recombinant full length protein (Human), MAD2L1.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: None
Constituents: Whole serum
Whole antiserum
Polyclonal
IgG
Cancer >> Cell cycle >> Cell division
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Other Nuclear Bodies
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Immunocytochemistry/ Immunofluorescence - MAD2 antibody (ab24588)
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Our Abpromise guarantee covers the use of ab24588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500Predicted molecular weight: 24 kDa.
IF: Use at an assay dependent dilution.
Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
Belongs to the MAD2 family.
Contains 1 HORMA domain.
The protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. The protein in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and of MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed form, and thereby promotes interaction with CDC20.
Phosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20.
Nucleus. Chromosome > centromere > kinetochore. Cytoplasm. Recruited by MAD1L1 to unattached kinetochores (Probable). Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD.
Target information above from: UniProt accessionQ13257
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Immunocytochemistry/ Immunofluorescence - MAD2 antibody (ab24588)

ab24588 at a 1/100 dilution staining human breast cancer cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 5% BSA prior to incubation with the antibody for 1 hour. A TRITC conjugated polyclonal anti-rabbit Ig was used as the secondary antibody. The image shows staining with ab24588 as well as a DNA stain. The right hand panel shows a merged image.
This image is courtesy of an Abreview submitted by Dr Lee Bergman
This product has been referenced in:
See all 3 publications for this product
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ab24588 at a 1/100 dilution staining human breast cancer cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 5% BSA prior to incubation with the antibody for 1 hour. A TRITC conjugated polyclonal anti-rabbit Ig was used as the secondary antibody. The image shows staining with ab24588 as well as a DNA stain. The right hand panel shows a merged image.
This image is courtesy of an Abreview submitted by Dr Lee Bergman
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