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Immunocytochemistry/ Immunofluorescence - MAD2 antibody (ab24588)

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Product Name
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Mad2L1 antibody
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See all Mad2L1 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Mad2L1
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Immunogen
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Recombinant full length protein (Human), MAD2L1.
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Reacts with
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Hu
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Tested applications
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions IF: Use at an assay dependent dilution. WB: 1/500. Predicted molecular weight: 24 kDa. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Cell cycle >> Cell division Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Other Nuclear Bodies Cell Biology >> Cell Cycle >> Cell Division >> Spindle
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Relevance
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Mad2L1 is required for the execution of the mitotic checkpoint which monitors the process of kinetochore spindle attachment and delays the onset of anaphase when this process is not complete. It inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
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Mad2L1 antibody (ab24588)
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: None Constituents: Whole serum
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Mad2L1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Mad2L1 antibody images, references, products related to ab24588 and other tools.
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Mad2L1 antibody images:
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Immunocytochemistry/ Immunofluorescence - MAD2 antibody (ab24588)
ab24588 at a 1/100 dilution staining human breast cancer cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 5% BSA prior to incubation with the antibody for 1 hour. A TRITC conjugated polyclonal anti-rabbit Ig was used as the secondary antibody. The image shows staining with ab24588 as well as a DNA stain. The right hand panel shows a merged image. This image is courtesy of an Abreview submitted by Dr Lee Bergman
See Abreview
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References for Mad2L1 antibody (ab24588)
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This product has been used in: (two most recent references)
Gisselsson D et al. When the genome plays dice: circumvention of the spindle assembly checkpoint and near-random chromosome segregation in multipolar cancer cell mitoses. PLoS ONE 3:e1871 (2008). ICC/IF; Human. PubMed: 18392149
Bentley AM et al. Distinct sequence elements of cyclin B1 promote localization to chromatin, centrosomes, and kinetochores during mitosis. Mol Biol Cell 18:4847-58 (2007). ICC/IF; Human. PubMed: 17881737
If you publish research using ab24588 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Mad2L1
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Mad2L1 antibody - more information
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Customer reviews (feedback) regarding Mad2L1 antibody |
Customer FAQs regarding Mad2L1 antibody |
Protocols for Mad2L1 antibody |
Price and availability of products related to Mad2L1 antibody |
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Mad2L1 antibody - related products:
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Mad2L1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Mad2L1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"