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All lanes : Anti-Mad2L1 antibody (ab3632) at 1/1200 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate with immunising peptide
Lane 4 : HeLa whole cell lysate with immunising peptide
Lysates/proteins at 35 µg per lane.
Secondary
IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 23.5 kDa
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
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