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Products:Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Overview
Product name
Anti-Mad2L2 antibody
See all Mad2L2 products (2) ...
Description
Rabbit polyclonal to Mad2L2
Specificity
This antibody detects a single band just below the 21kD marker, that corresponds to Mad2L2 / Mad2B.
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Xenopus laevis
Immunogen
Synthetic peptide: TLTRQDLNFGQV, corresponding to amino acids 3-14 of Human Mad2L2.
TLTRQDLNFG QV
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Cell division
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
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Western blot - Mad2L2 antibody (ab3630)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: 1/500. Detects a band of approximately ~20 kDa (predicted molecular weight: 24 kDa). Can be blocked with Mad2L2 (aa 3-14) peptide (human)
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
MAD2L2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L2 is a homolog of MAD2L1.
Cellular localization
Nuclear
Alternative names
- Homolog of REV7 S cerevisiae antibody
- HREV7 antibody
- MAD2 homolog antibody
see all
Database links
- Clone ID: BC015244 Human
- Entrez Gene: 10459 Human
- GenBank: NM_006341 Human
- IMAGE Id: 3916742 Human
- Omim: 604094 Human
- SwissProt: Q9UI95 Human
- Unigene: 19400 Human
Anti-Mad2L2 antibody images:
Western blot - Mad2L2 antibody (ab3630)
All lanes : Anti-Mad2L2 antibody (ab3630) at 1/500 dilution
Lane 1 : Whole cell extract prepared from Hela cells
Lane 2 : Nuclear lysates prepared from Hela cells
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/2000 dilution
Predicted band size : 24 kDa
References for Anti-Mad2L2 antibody (ab3630)
ab3630 has not yet been referenced specifically in any publications.
Publishing research using ab3630? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"