Anti-MafA antibody (ab17976)
Key features and details
- Rabbit polyclonal to MafA
- Suitable for: WB, EMSA
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MafA antibody
See all MafA primary antibodies -
Description
Rabbit polyclonal to MafA -
Host species
Rabbit -
Tested applications
Suitable for: WB, EMSAmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Mouse MafA (C terminal).
Database link: Q8CF90 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000.
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EMSA |
Use a concentration of 0.05 - 0.25 mg/ml.
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Notes |
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WB
1/1000 - 1/10000. |
EMSA
Use a concentration of 0.05 - 0.25 mg/ml. |
Target
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Function
Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. -
Sequence similarities
Belongs to the bZIP family. Maf subfamily.
Contains 1 bZIP domain. -
Post-translational
modificationsUbiquitinated, leading to its degradation by the proteasome.
Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through an ubiquitin/proteasome-dependent pathway. -
Cellular localization
Nucleus. Detected in nuclei of pancreas islet beta cells. - Information by UniProt
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Database links
- Entrez Gene: 389692 Human
- Entrez Gene: 366949 Rat
- Omim: 610303 Human
- SwissProt: Q8NHW3 Human
- Unigene: 521914 Human
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Alternative names
- HMafA antibody
- Mafa antibody
- MAFA_HUMAN antibody
see all
Images
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Detection of MafA, MafB and cMaf by Western Blot. Samples: Nuclear extract (6 µg) from HeLa cells transfected with MafA, MafB or cMaf expression constructs. Antibodies: Affinity purified anti-MafA antibody, anti-MafB Antibody or anti-c-Maf antibody. Each antibody was used at 0.5 µg/ml. Detection: Chemiluminescence with a 5 second exposure.
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Electrophoretic Mobility Shift of MafA, MafB and c-Maf. Samples: Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or c-Maf expression constructs. Antibodies: Affinity purified anti-MafA antibody BL1069, anti-MafB Antibody or anti-c-Maf antibody.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab17976 has been referenced in 5 publications.
- Xu J et al. MAFB mediates the therapeutic effect of sleeve gastrectomy for obese diabetes mellitus by activation of FXR expression. Braz J Med Biol Res 51:e7312 (2018). PubMed: 29846411
- Roth Flach RJ et al. Protein Kinase Mitogen-activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) Promotes Obesity-induced Hyperinsulinemia. J Biol Chem 291:16221-30 (2016). IHC-P ; Mouse . PubMed: 27226575
- Sakano D et al. VMAT2 identified as a regulator of late-stage ß-cell differentiation. Nat Chem Biol 10:141-8 (2014). PubMed: 24316738
- Hunter KE et al. Identification and characterization of poorly differentiated invasive carcinomas in a mouse model of pancreatic neuroendocrine tumorigenesis. PLoS One 8:e64472 (2013). WB, IHC-P ; Mouse . PubMed: 23691228
- Vargas N et al. TAT-Mediated Transduction of MafA Protein In Utero Results in Enhanced Pancreatic Insulin Expression and Changes in Islet Morphology. PLoS One 6:e22364 (2011). WB ; Mouse . PubMed: 21857924