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Western blot - MafB antibody (ab56242)
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Immunohistochemistry (Paraffin-embedded sections) - MafB antibody (ab56242)
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Product Name
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MafB antibody
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See all MafB antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MafB
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Immunogen
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Synthetic peptide corresponding to a region of Human MAFB
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Reacts with
(species key)
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Hu, Rat, Zfsh
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a concentration of 4.0 - 8.0 µg/ml.
WB: Use at a concentration of 0.25 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 36 kDa).
ELISA titre using peptide based assay: 1/62500
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HepG2 cell lysate; human spleen tissue
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
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Relevance
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MAF family members, such as MAFB, are basic region/leucine zipper transcription factors that regulate transcription positively or negatively, depending on their partner proteins and the context of the target promoter. MafB plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MafB antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MafB antibody images, references, products related to ab56242 and other tools.
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MafB antibody images:
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 Western blot - MafB antibody (ab56242)
MafB antibody (ab56242) at 0.25 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP-conjugated anti-rabbit IgG at 1/50000 dilution
Predicted band size : 36 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
Gel concentration 12%
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Immunohistochemistry (Paraffin-embedded sections) - MafB antibody (ab56242)
Immunohistochemical analysis of paraffin-embedded human spleeen tissue using ab56242 at a dilution of 4-8 µg/ml, positively labelling spleen cells (indicated with arrows). Magnification 400X
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Search PubMed (MEDLINE) for references to MafB
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MafB antibody - more information
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Customer reviews (feedback) regarding MafB antibody |
Customer FAQs regarding MafB antibody |
Protocols for MafB antibody |
Price and availability of products related to MafB antibody |
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MafB antibody - related products:
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MafB antibody - other tools:
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Contact Abcam with a Technical Enquiry about MafB antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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