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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Overview
Product name
Anti-MafB antibody
See all MafB products (4) ...
Description
Rabbit polyclonal to MafB
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Zebrafish
Immunogen
Synthetic peptide corresponding to a region of Human MAFB
Positive control
HepG2 cell lysate; human spleen tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab56242 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.25 µg/mlDetects a band of approximately 32 kDa (predicted molecular weight: 36 kDa).
IHC-P: Use a concentration of 4.0 - 8.0 µg/ml.
Target
Relevance
MAF family members, such as MAFB, are basic region/leucine zipper transcription factors that regulate transcription positively or negatively, depending on their partner proteins and the context of the target promoter. MafB plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells.
Cellular localization
Nuclear
Alternative names
- Kreisler antibody
- Kreisler (mouse) maf related leucine zipper homolog antibody
- Kreisler maf related leucine zipper homolog antibody
see all
Database links
- Entrez Gene: 9935 Human
- Entrez Gene: 16658 Mouse
- Entrez Gene: 54264 Rat
- GenBank: AF134157.1 Human
- Omim: 608968 Human
- SwissProt: Q9Y5Q3 Human
- SwissProt: P54841 Mouse
- SwissProt: P54842 Rat
see all
Anti-MafB antibody images:
Western blot - MafB antibody (ab56242)
Anti-MafB antibody (ab56242) at 0.25 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP-conjugated anti-rabbit IgG at 1/50000 dilution
Predicted band size : 36 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
Gel concentration 12%
Immunohistochemistry (Paraffin-embedded sections) - MafB antibody (ab56242)
Immunohistochemical analysis of paraffin-embedded human spleeen tissue using ab56242 at a dilution of 4-8 µg/ml, positively labelling spleen cells (indicated with arrows). Magnification 400X
References for Anti-MafB antibody (ab56242)
ab56242 has not yet been referenced specifically in any publications.
Publishing research using ab56242? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"