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Western blot - MafB antibody (ab65953)
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Immunocytochemistry/ Immunofluorescence - MafB antibody (ab65953)
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Product Name
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MafB antibody
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See all MafB antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MafB
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 250 - 350 MafB. (Peptide available as ab71662.)
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Reacts with
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Hu, Rat
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Predicted to react
(species key)
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Ms, NHuPrm
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following lysates:
HeLa Whole Cell, HepG2 Whole Cell, Caco 2 Whole Cell, Rat Thymus Tissue (data not shown), Rat Colon Tissue (data not shown)
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
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Relevance
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MAF family members, such as MAFB, are basic region/leucine zipper transcription factors that regulate transcription positively or negatively, depending on their partner proteins and the context of the target promoter. MafB plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MafB antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MafB antibody images, references, products related to ab65953 and other tools.
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MafB antibody images:
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 Western blot - MafB antibody (ab65953)
All lanes : MafB antibody (ab65953) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP)
at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 36 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Additional bands at : 105 kDa,55 kDa,63 kDa. We are unsure as to the identity of these extra bands.
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Immunocytochemistry/ Immunofluorescence - MafB antibody (ab65953)
ICC/IF image of ab65953 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65953, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 cells at 5µg/ml.
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Search PubMed (MEDLINE) for references to MafB
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MafB antibody - more information
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Customer reviews (feedback) regarding MafB antibody |
Customer FAQs regarding MafB antibody |
Protocols for MafB antibody |
Price and availability of products related to MafB antibody |
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MafB antibody - related products:
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MafB antibody - other tools:
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Contact Abcam with a Technical Enquiry about MafB antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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