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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Anti-MafB antibody
See all MafB products (4) ...
Rabbit polyclonal to MafB
IHC-FoFr, WB, IHC-P, Flow Cyt, ICC/IFmore details
Reacts with
Mouse, Rat, Human
Predicted to work with
Chicken, Zebrafish
Synthetic peptide: AAELSMGPEL PTSPLAMEYV NDFDLLKFDV KKEPLGRAER PGRPCTRLQP , corresponding to amino acids 2-51 of Human MafB
AAELSMGPEL PTSPLAMEYV NDFDLLKFDV KKEPLGRAER PGRPCTRLQP
WB: HepG2 cell lysate IHC-P: Human kidney tissue
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
Polyclonal
IgG
Our Abpromise guarantee covers the use of ab66506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr: Use at an assay dependent dilution. (PubMed: 20436469)
WB: Use a concentration of 1.25 µg/mlDetects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-P: Use a concentration of 4 - 8 µg/ml.
Flow Cyt: Use 4µg for 106 cells.
ICC/IF: Use at an assay dependent dilution.
MAF family members, such as MAFB, are basic region/leucine zipper transcription factors that regulate transcription positively or negatively, depending on their partner proteins and the context of the target promoter. MafB plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells.
Nuclear
Western blot - MafB antibody (ab66506)

Anti-MafB antibody (ab66506) at 1.25 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 36 kDa
Observed band size : 36 kDa
Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MafB antibody (ab66506)

ab66506, at 8µg/ml, staining MafB in epithelial cells of paraffin embedded human kidney renal tubule (indicated by arrows) by Immunohistochemistry.
Flow Cytometry - MafB antibody (ab66506)

ab66506, at 4 µg/cells, staining MAFB in mouse bone marrow by flow cytometry. Cells were paraformaldehyde fixed and permeabilized using Saponin. The secondary antibody was ab6717, a FITC-conjugated goat polyclonal to rabbit IgG.
This image is courtesy of an Abreview submitted by Kay Muthu.
This product has been referenced in:
See all 2 publications for this product
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Anti-MafB antibody (ab66506) at 1.25 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 36 kDa
Observed band size : 36 kDa
Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.

ab66506, at 8µg/ml, staining MafB in epithelial cells of paraffin embedded human kidney renal tubule (indicated by arrows) by Immunohistochemistry.

ab66506, at 4 µg/cells, staining MAFB in mouse bone marrow by flow cytometry. Cells were paraformaldehyde fixed and permeabilized using Saponin. The secondary antibody was ab6717, a FITC-conjugated goat polyclonal to rabbit IgG.
This image is courtesy of an Abreview submitted by Kay Muthu.
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