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Western blot - MafB antibody (ab66506)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MafB antibody (ab66506)
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Flow Cytometry - MafB antibody (ab66506)
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Product Name
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MafB antibody
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See all MafB antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MafB
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Immunogen
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Synthetic peptide: AAELSMGPEL PTSPLAMEYV NDFDLLKFDV KKEPLGRAER PGRPCTRLQP , corresponding to amino acids 2-51 of Human MafB
BLAST 'AAELSMGPEL PTSPLAMEYV NDFDLLKFDV KKEPLGRAER PGRPCTRLQP ' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ELISA, Flow Cyt, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA titre using peptide based assay: 1/312500.
Flow Cyt: Use 4 µg for 106 cells.
ICC/IF: Use at an assay dependent concentration.
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: HepG2 cell lysate
IHC-P: Human kidney tissue
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
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Relevance
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MAF family members, such as MAFB, are basic region/leucine zipper transcription factors that regulate transcription positively or negatively, depending on their partner proteins and the context of the target promoter. MafB plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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2.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MafB antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MafB antibody images, references, products related to ab66506 and other tools.
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MafB antibody images:
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 Western blot - MafB antibody (ab66506)
MafB antibody (ab66506) at 1.25 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 36 kDa
Observed band size : 36 kDa
Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MafB antibody (ab66506)
ab66506, at 8µg/ml, staining MafB in epithelial cells of paraffin embedded human kidney renal tubule (indicated by arrows) by Immunohistochemistry.
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Flow Cytometry - MafB antibody (ab66506)
ab66506, at 4 µg/cells, staining MAFB in mouse by flow cytometry.
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References for MafB antibody (ab66506)
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This product has been used in:
Smink JJ et al. Transcription factor C/EBPbeta isoform ratio regulates osteoclastogenesis through MafB. EMBO J 28:1769-81 (2009). WB; Mouse. PubMed: 19440205
If you publish research using ab66506 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MafB
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MafB antibody - more information
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Customer reviews (feedback) regarding MafB antibody |
Customer FAQs regarding MafB antibody |
Protocols for MafB antibody |
Price and availability of products related to MafB antibody |
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MafB antibody - related products:
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MafB antibody - other tools:
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Contact Abcam with a Technical Enquiry about MafB antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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