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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Overview
Product name
Anti-MafB antibody
See all MafB products (4) ...
Description
Rabbit polyclonal to MafB
Tested applications
IHC-FoFr, WB, IHC-P, Flow Cyt, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Chicken, Zebrafish
Immunogen
Synthetic peptide: AAELSMGPEL PTSPLAMEYV NDFDLLKFDV KKEPLGRAER PGRPCTRLQP , corresponding to amino acids 2-51 of Human MafB
AAELSMGPEL PTSPLAMEYV NDFDLLKFDV KKEPLGRAER PGRPCTRLQP
Positive control
WB: HepG2 cell lysate IHC-P: Human kidney tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr: Use at an assay dependent dilution. (PubMed: 20436469)
WB: Use a concentration of 1.25 µg/mlDetects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-P: Use a concentration of 4 - 8 µg/ml.
Flow Cyt: Use 4µg for 106 cells.
ICC/IF: Use at an assay dependent dilution.
Target
Relevance
MAF family members, such as MAFB, are basic region/leucine zipper transcription factors that regulate transcription positively or negatively, depending on their partner proteins and the context of the target promoter. MafB plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells.
Cellular localization
Nuclear
Alternative names
- Kreisler antibody
- Kreisler (mouse) maf related leucine zipper homolog antibody
- Kreisler maf related leucine zipper homolog antibody
see all
Database links
- Entrez Gene: 9935 Human
- Entrez Gene: 16658 Mouse
- Entrez Gene: 54264 Rat
- GenBank: AF134157.1 Human
- Omim: 608968 Human
- SwissProt: Q9Y5Q3 Human
- SwissProt: P54841 Mouse
- SwissProt: P54842 Rat
see all
Anti-MafB antibody images:
Western blot - MafB antibody (ab66506)
Anti-MafB antibody (ab66506) at 1.25 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 36 kDa
Observed band size : 36 kDa
Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MafB antibody (ab66506)
ab66506, at 8µg/ml, staining MafB in epithelial cells of paraffin embedded human kidney renal tubule (indicated by arrows) by Immunohistochemistry.
Flow Cytometry - MafB antibody (ab66506)
ab66506, at 4 µg/cells, staining MAFB in mouse bone marrow by flow cytometry. Cells were paraformaldehyde fixed and permeabilized using Saponin. The secondary antibody was ab6717, a FITC-conjugated goat polyclonal to rabbit IgG.
This image is courtesy of an Abreview submitted by Kay Muthu.
References for Anti-MafB antibody (ab66506)
This product has been referenced in:
- Beaty THet al. A genome-wide association study of cleft lip with and without cleft palate identifies risk variants near MAFB and ABCA4. Nat Genet 42:525-9 (2010). IHC-FoFr; Mouse.Read more (PubMed: 20436469) »
- Smink JJet al. Transcription factor C/EBPbeta isoform ratio regulates osteoclastogenesis through MafB. EMBO J 28:1769-81 (2009). WB; Mouse.Read more (PubMed: 19440205) »
See all 2 publications for this product
Publishing research using ab66506? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"