Feedback Form

Western blot - MafB antibody (ab66506)
Western blot - MafB antibody (ab66506)
(enlarge)


Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MafB antibody (ab66506)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MafB antibody (ab66506)
(enlarge)


Flow Cytometry - MafB antibody (ab66506)
Flow Cytometry - MafB antibody (ab66506)
(enlarge)


Product Name 

MafB antibody

 

See all MafB antibodies (4)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MafB

Immunogen 

Synthetic peptide: AAELSMGPEL PTSPLAMEYV NDFDLLKFDV KKEPLGRAER PGRPCTRLQP , corresponding to amino acids 2-51 of Human MafB
BLAST 'AAELSMGPEL PTSPLAMEYV NDFDLLKFDV KKEPLGRAER PGRPCTRLQP ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

ELISA, Flow Cyt, ICC/IF, IHC-P, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews
(Read all Abreviews)

Submit an Abreview and earn up to 220 Abpoints by reviewing this product

• Flow Cyt

1 Abreview

- average rating: 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)

• ICC/IF

1 Abreview

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)


ICC/IF
(read Abreview)


Flow Cyt
(read Abreview)


Application notes 
(see key)

Recommended dilutions
ELISA titre using peptide based assay: 1/312500.
Flow Cyt: Use 4 µg for 106 cells.
ICC/IF: Use at an assay dependent concentration.
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: HepG2 cell lysate
IHC-P: Human kidney tissue

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper

Relevance 

MAF family members, such as MAFB, are basic region/leucine zipper transcription factors that regulate transcription positively or negatively, depending on their partner proteins and the context of the target promoter. MafB plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells.

 

Alternative names MafB antibody (ab66506)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

9935    (Human)
16658    (Mouse)
54264    (Rat)

GenBank

    

AF134157.1    (Human)

Omim

    

608968    (Human)

SwissProt

    

Q9Y5Q3    (Human)
P54841    (Mouse)
P54842    (Rat)

Unigene

    

651210     (Human)
10725    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Form 

Liquid

Concentration 

2.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MafB antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for MafB antibody images, references, products related to ab66506 and other tools.

 

MafB antibody images:

Product image  Western blot - MafB antibody (ab66506)

Western blot - MafB antibody (ab66506)

MafB antibody (ab66506) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 36 kDa
Observed band size : 36 kDa
Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.

 

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MafB antibody (ab66506)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MafB antibody (ab66506)

ab66506, at 8µg/ml, staining MafB in epithelial cells of paraffin embedded human kidney renal tubule (indicated by arrows) by Immunohistochemistry.

 

Product image  Flow Cytometry - MafB antibody (ab66506)

Flow Cytometry - MafB antibody (ab66506)

ab66506, at 4 µg/cells, staining MAFB in mouse by flow cytometry.

 

References for MafB antibody (ab66506)

Specific publication references   This product has been used in:

Smink JJ et al. Transcription factor C/EBPbeta isoform ratio regulates osteoclastogenesis through MafB. EMBO J 28:1769-81 (2009). WB; Mouse. PubMed: 19440205

If you publish research using ab66506 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to MafB

 

MafB antibody - more information

Customer reviews (feedback) regarding MafB antibody

Customer FAQs regarding MafB antibody

Protocols for MafB antibody

Price and availability of products related to MafB antibody

 

MafB antibody - related products:

Products for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Slides

Products for Western blot

Loading controls

Lysates

 

Positive Controls for MafB antibody

ab14660

  

HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate

ab4347

  

Kidney (human) normal tissue slides

 

Compatible Secondaries for MafB antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other MafB Antibodies

See all MafB antibodies (4)...

 

MafB antibody - other tools:

Contact Abcam with a Technical Enquiry about MafB antibody

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.