You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-MafB antibody (ab82837)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-MafB antibody
See all MafB products (4) ...

Description

Rabbit polyclonal to MafB

Tested applications

ELISA, WBmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

3 synthetic peptides (human) conjugated to KLH

Positive control

Induced culture of E.coli (one shot Top10 competent cells). Plasmid name: pBAD-DEST49.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Thimerosal (merthiolate)
Constituents: Whole serum

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Crude serum, final bleed.

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper

  • Western blot - MafB antibody (ab82837)Western blot - MafB antibody (ab82837) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab82837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution.
Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/20000 is determined for one of the three peptides.
WB: 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

MAF family members, such as MAFB, are basic region/leucine zipper transcription factors that regulate transcription positively or negatively, depending on their partner proteins and the context of the target promoter. MafB plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells.

Cellular localization

Nuclear

Alternative names

  • Kreisler antibody
  • Kreisler (mouse) maf related leucine zipper homolog antibody
  • Kreisler maf related leucine zipper homolog antibody
  • KRML antibody
  • MAFB/Kreisler basic region/leucine zipper transcription factor antibody
  • MGC43127 antibody
  • Segmentation protein KR antibody
  • Transcription factor MafB antibody
  • V maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian) antibody
see all

Database links

Anti-MafB antibody images:

  Western blot - MafB antibody (ab82837)

Western blot - MafB antibody (ab82837)

All lanes : Anti-MafB antibody (ab82837) at 1/500 dilution

Lane 1 : uninduced culture of E.coli (one shot Top10 competent cells)
Lane 2 : induced culture of E.coli (one shot Top10 competent cells)

Secondary
anti-Rabbit IgG HRP at 1/1000 dilution

Predicted band size : 50 kDa
Observed band size : 50 kDa


Predicted molecular weight is 35.7kDa, tag is 14kDa.

References for Anti-MafB antibody (ab82837)

ab82837 has not yet been referenced specifically in any publications.

Publishing research using ab82837? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"