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Magnesium Detection Kit (ab102506)

Q&A (5)

Overview

  • Product nameMagnesium Detection Kit
  • Tests
    100 x 1 assay
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay typeQuantitative
  • Sensitivity
    40 µM
  • Range
    2 µM - 15 µM
  • Assay time
    0h 30m
  • Product overview

    Abcam's Magnesium Assay Kit provides a simple sensitive means of quantitating magnesium in a variety of biological samples. The kit takes advantage of the specific requirement of glycerol kinase for Mg2+. An enzyme linked reaction leads to formation of an intensely colored (λmax = 450nm) product whose formation is proportional to Mg2+ concentration. The linear range of the assay is 2-15 nmoles with detection sensitivity ~40 µM.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Magnesium is the 11th most abundant element by mass in the human body. Mg2+ is essential to all living cells where it plays an important role in facilitating the processing of biological polyphosphates like ATP, DNA, RNA and enzyme functions. Mg2+ is the metallic ion at the center of chlorophyll, and a common additive to fertilizers. Mg2+ compounds are used as laxatives, antacids, and used to stabilize abnormal nerve excitation and blood vessel spasm i.e., eclampsia.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceMagnesium is the 11th most abundant element by mass in the human body. Magnesium is essential to all living cells where it plays an important role in facilitating the processing of biological polyphosphates like ATP, DNA, RNA and enzyme functions. Magnesium is the metallic ion at the center of chlorophyll, and a common additive to fertilizers. Magnesium compounds are used as laxatives, antacids, and used to stabilize abnormal nerve excitation and blood vessel spasm i.e., eclampsia.
    • Alternative names
        Mg++Mg+2Mg2+

    Applications

    Our Abpromise guarantee covers the use of ab102506 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Magnesium Detection Kit images

    • Functional Studies - Magnesium Detection Kit (ab102506)
      Functional Studies - Magnesium Detection Kit (ab102506)
      Magnesium standard curve: Assay is performed according to kit protocol. Vertical dotted lines indicate the lower and upper limits of normal serum Magnesium concentrations.

    Protocols

    References for Magnesium Detection Kit (ab102506)

    ab102506 has not yet been referenced specifically in any publications.

    Publishing research using ab102506 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

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    Displaying 1 - 5 of 5 results for Abreviews and Q&A

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    I tried another standard and set of reagents today and the assay worked fine! Thank you for your help trying to resolve this.

    I am happy to hear that the kit is working for you! Please do not hesitate to contact me if I can be of any further assistance.

    The enzyme mixture was reconstituted in 550 uL of assay buffer. It was stored at -20 C.

    The standards were prepared by mixing 10 uL of magnesium standard with 990 uL of water. The remaining standards were diluted down from that stock solution.

    Thank you for your reply.


    We have rechecked the QC and retested the kit which is working as expected, therefore it is unclear why the kit is not working well in your hands. As this kit is covered by the Abpromise I am happy to offer a repl...

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    The kit was reconstituted 17-FEB-2012. I believe it was received a few days before that. The curve was repeated the following day with similar results. Once the enzyme mixture was added to the samples, all of the standards turned color at the same relativ...

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    Thank you for your reply.


    The lab has some additional questions: How was theenzyme reconstituted/stored? Can you also describe how standards were prepared?


    We want to be sure we cover all possibilities since the QC looks gr...

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    Thank you for your help. Here is the excel file for the data we generated. This was a standard curve. 1.1 mL of water was used to reconstitute the developer and 550 uL was used to reconstitute the enzyme. The rest of the protocol was followed. We repeated...

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    Thank you for your patience as I have investigated this problem further.


    Based on the data you show it looks like the issue is thatno reaction mix or old/expired mix was used as there is not even the hint of a difference between 0 and the ...

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    I've followed the protocol, but I'm seeing a "flat" standard curve.

    I am sorry to hear that this kit is not providing satisfactory results.

    Could you please send me the data in an Excel format so we may take a look and make any suggestions as necessary? The details provided will enable us to investigate this ca...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"