You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameMalate Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Milk, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    20 µM
  • Range
    1 µM - 35 µM
  • Assay time
    1h 00m
  • Product overview

    Abcam's Malate Assay Kit is an easy and sensitive assay to measure the L(-) Malate level in a variety of samples. In the assay, malate is specifically oxidized to generate a product which reacts with a substrate probe to generate color (λmax = 450 nm). The assay can detect ~1-35 nmol of Malate in a 50 µl sample with a detection sensitivity ~20 µM.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    L(-) Malate is a TCA cycle intermediate. It plays an important role in the Calvin cycle during carbon fixation in plants. In lower organisms, malate is converted to lactate during malolactic fermentation with the formation of CO2. Malate is frequently used as an additive in the food and pharmaceutical industries, so quantitating malic acid is important in manufacturing beer, wine, cheese and fruits, among others.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceL(-) Malate is a TCA cycle intermediate. It plays an important role in the Calvin cycle during carbon fixation in plants. In lower organisms, malate is converted to lactate during malolactic fermentation with the formation of CO2. Malate is frequently used as an additive in the food and pharmaceutical industries, so quantitating malic acid is important in manufacturing beer, wine, cheese and fruits, among others.
    • Alternative names
        Malic acid

    Applications

    Our Abpromise guarantee covers the use of ab83391 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Malate Assay Kit images

    • Functional Studies - Malate Assay Kit (ab83391)
      Functional Studies - Malate Assay Kit (ab83391)
      L(-) Malate standard curve generated using ab83391.

    Protocols

    References for Malate Assay Kit (ab83391)

    This product has been referenced in:
    • Liu J  et al. Roles of AtpI and two YidC-type proteins from alkaliphilic Bacillus pseudofirmus OF4 in ATP synthase assembly and nonfermentative growth. J Bacteriol 195:220-30 (2013). Read more (PubMed: 23123906) »

    See 1 Publication for this product

    Publishing research using ab83391 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit a Question for ab83391

    Displaying 1 - 7 of 7 results for Q&A

    10
    Votes: Highest First

    Hello, I need a malate assay for bacteria (legionella). Do you know if I could use some of yours products? Thanks

    Thank you for contacting us.

    The product referred in your email (ab119693) is not intended to measure Malate in your samples, but the enzymatic activity of Malate dehydrogenase.

    We do have a different kit in the catalogue, ab83391, whi...

    Read More

    I have several questions about this kit. One is that I notice that the buffer foams easily. I assume it has detergent in it. The bubbles resulting from pipetting the buffer interfere with accurate measurements of the absorbance at 450 nm using our plate r...

    Read More

    Thank you for contacting us.

    Yes, this assay buffer had detergents and any bubbles will interfere with the readings. Please take care when pipeting to avoid bubbles. Should you have many bubbles please try to remove these from the well.
    ...

    Read More

    I need to know whether the kits (color change) is based on product formed or product depleted?

    Thank you for your enquiry. Here are the details you requested. 1) Malate Assay Kit (ab83391): detects product formed (expected delivery date9th Jan) Step 1: Malate in sample + oxidation = product formed Step 2: Product formed + probe = color generation ...

    Read More

    Happy New Year 2012 to you all .. I had a discussion with my boss today regarding the kits that you have offered. I would like to know following things.. · I understand that the kit is not able to directly measure the enzyme activity. However, we ...

    Read More

    Thanks for contacting me. I have looked at these kits that we discussed and here are my thoughts: Malate Assay Kit (ab83391): This kit will only measure malate present in the sample. The enzyme used to produce the oxidized form of malate, the form that is...

    Read More

    I am looking for enzyme assay kits that can measure enzyme activity (not the amount of enzyme in the sample). Sample is from soil microbs, will extract the enzyme and detect using enzyme kits.

    Thank you for contacting us the other day. I have looked at the list of kits (attached file, summary that my colleague sent previously) that you are looking for and unfortunately, as my colleague has informed you, we only have 3 kits that may or may n...

    Read More

    I want to use cultured cells for ab83391, Malate Assay Kit. Can I use cultured cells as sample? How should I prepare the cells for the kit?

    Thank you for your enquiry. Yes, you can use the cultured cells for ab83391. Here is the recommended sample preparation method for cultured cells; Cells (1-5X10e6) should be rapidly homogenized with two volumes of ice cold PBS or other buffer (pH 6.5-...

    Read More

    Dear Sir or Madam, I am sending this e-mail to enquire about technical information of three kits from abcam: Malate Assay kit (ab83391) Isocitrate Assay kit (ab83424) Citrate Assay kit (ab83396) I need to measure these compounds in blood s...

    Read More

    Thank you for your enquiry. I would be pleased to help answer your questions. 1. I can confirm you should be able to use these kits with blood samples. Generally serum or plasma that is obtained from blood samples is used for such purposes. However ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"