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Abcam's Malate Assay Kit is an easy and sensitive assay to measure the L(-) Malate level in a variety of samples. In the assay, malate is specifically oxidized to generate a product which reacts with a substrate probe to generate color (?max = 450 nm). The assay can detect ~1-35 nmol of Malate in a 50 µl sample with a detection sensitivity ~20 µM.
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L(-) Malate is a TCA cycle intermediate. It plays an important role in the Calvin cycle during carbon fixation in plants. In lower organisms, malate is converted to lactate during malolactic fermentation with the formation of CO2. Malate is frequently used as an additive in the food and pharmaceutical industries, so quantitating malic acid is important in manufacturing beer, wine, cheese and fruits, among others.
|Malate assay buffer||WM||1 x 20ml|
|Malate Enzyme Mix (Lyophilised)||Green||1 vial|
|Malate Standard (10 µmol) (Lyophilized)||Yellow||1 vial|
|WST Substrate (Lyophilised)||Red||1 vial|
Our Abpromise guarantee covers the use of ab83391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|