Overview

  • Product nameMalate Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Milk, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    20 µM
  • Range
    1 µM - 35 µM
  • Assay time
    1h 00m
  • Product overview

    Abcam's Malate Assay Kit is an easy and sensitive assay to measure the L(-) Malate level in a variety of samples. In the assay, malate is specifically oxidized to generate a product which reacts with a substrate probe to generate color (λmax = 450 nm). The assay can detect ~1-35 nmol of Malate in a 50 µl sample with a detection sensitivity ~20 µM.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    L(-) Malate is a TCA cycle intermediate. It plays an important role in the Calvin cycle during carbon fixation in plants. In lower organisms, malate is converted to lactate during malolactic fermentation with the formation of CO2. Malate is frequently used as an additive in the food and pharmaceutical industries, so quantitating malic acid is important in manufacturing beer, wine, cheese and fruits, among others.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceL(-) Malate is a TCA cycle intermediate. It plays an important role in the Calvin cycle during carbon fixation in plants. In lower organisms, malate is converted to lactate during malolactic fermentation with the formation of CO2. Malate is frequently used as an additive in the food and pharmaceutical industries, so quantitating malic acid is important in manufacturing beer, wine, cheese and fruits, among others.
  • Alternative names
    • Malic acid

Applications

Our Abpromise guarantee covers the use of ab83391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Functional Studies Use at an assay dependent dilution.

Malate Assay Kit images

Protocols

References for Malate Assay Kit (ab83391)

This product has been referenced in:
  • Liu J  et al. Roles of AtpI and two YidC-type proteins from alkaliphilic Bacillus pseudofirmus OF4 in ATP synthase assembly and nonfermentative growth. J Bacteriol 195:220-30 (2013). Read more (PubMed: 23123906) »

See 1 Publication for this product

Product Wall

Thank you for contacting us.

The product referred in your email (ab119693) is not intended to measure Malate in your samples, but the enzymatic activity of Malate dehydrogenase.

We do have a different kit in the catalogue, ab83391, ...

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Yes, this assay buffer had detergents and any bubbles will interfere with the readings. Please take care when pipeting to avoid bubbles. Should you have many bubbles please try to remove these from the well. Read More

Thank you for your enquiry. Here are the details you requested. 1) Malate Assay Kit (ab83391): detects product formed (expected delivery date9th Jan) Step 1: Malate in sample + oxidation = product formed Step 2: Product formed + probe = color generati...

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Thanks for contacting me. I have looked at these kits that we discussed and here are my thoughts: Malate Assay Kit (ab83391): This kit will only measure malate present in the sample. The enzyme used to produce the oxidized form of malate, the form that...

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Thank you for contacting us the other day. I have looked at the list of kits (attached file, summary that my colleague sent previously) that you are looking for and unfortunately, as my colleague has informed you, we only have 3 kits that may or ma...

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Thank you for your enquiry. Yes, you can use the cultured cells for ab83391. Here is the recommended sample preparation method for cultured cells; Cells (1-5X10e6) should be rapidly homogenized with two volumes of ice cold PBS or other buffer (pH 6...

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Thank you for your enquiry. I would be pleased to help answer your questions. 1. I can confirm you should be able to use these kits with blood samples. Generally serum or plasma that is obtained from blood samples is used for such purposes. Howev...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"