Maleimide Quantification Assay Kit (Colorimetric) (ab112140)

Overview

  • Product nameMaleimide Quantification Assay Kit (Colorimetric)See all Maleimide kits ...
  • Tests
    1 x 100 test
  • Sample type
    Cell culture extracts, Adherent cells, Suspension cells
  • Assay typeQuantitative
  • Sensitivity
    10 pg/well
  • Product overview

    ab112140 assay kit quantifies maleimide groups by first reacting a sample with a known amount of thiol present in excess and then assaying the remaining unreacted thiol using 4,4'-DTDP with a molar extinction coefficient of 19,800 M -1cm-1. The amount of maleimide is calculated as the difference between the initial amount of thiol and the amount of unreacted thiol after the complete reaction of all maleimide groups. This spectrophotometric assay for the determination of maleimide groups is a reverse GSH assay. It takes advantage of the high reactivity of thiols of GSH with the maleimide moiety. Maleimide of the sample is allowed to form a stable thiosuccinimidyl linkage with GSH. After the reaction of the sample is complete, the excess GSH, i.e., the remaining thiols of GSH in the reaction mixture, is estimated by using 4,4'-DTDP. The amount of GSH reacted with the sample is titrated to determine the extent of maleimide. For more sensitive maleimide quantitation, we recommend that you use our fluorimetric kit ab112141 that has higher sensitivity.

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  • Tested applicationsFunctional Studies more details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab112140 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies Use at an assay dependent concentration.

    Protocols

    References for Maleimide Quantification Assay Kit (Colorimetric) (ab112140)

    ab112140 has not yet been referenced specifically in any publications.

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