Maleimide Quantification Assay Kit (Fluorometric) (ab112141)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
Overview
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Product name
Maleimide Quantification Assay Kit (Fluorometric)
See all Maleimide kits -
Detection method
Fluorescent -
Assay type
Quantitative -
Product overview
ab112141 uses a proprietary dye that has enhanced fluorescence upon reacting with a maleimide. The kit provides a sensitive, one-step fluorometric method to detect as little as 10 picomoles of maleimide in a 100 µL assay volume (100 nM; Figure 1). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 490/520 nm. Compared to ab112140, this fluorometric assay is more sensitive, and has less interference from biological samples.
Visit our FAQs page for tips and troubleshooting.
Please note this kit is optimized for the detection of small molecule-based maleimide.
If your sample is maleimide conjugated in protein, then this kit may not be suitable.
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Notes
ab112141 should be stored desiccated.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 tests Assay Buffer 1 x 25ml DMSO 1 x 200µl MalemGreen indicator 1 vial N-ethylmaleimide Standard 1 x 50µl Reaction Buffer 1 x 500µl -
Research areas
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab112141 has been referenced in 2 publications.
- Hurst CH et al. Maleimide scavenging enhances determination of protein S-palmitoylation state in acyl-exchange methods. Biotechniques 62:69-75 (2017). PubMed: 28193150
- Valetti S et al. Peptide conjugation: before or after nanoparticle formation? Bioconjug Chem 25:1971-83 (2014). PubMed: 25313527