Maleimide Quantification Assay Kit (Fluorometric) (ab112141)
- Product nameMaleimide Quantification Assay Kit (Fluorometric)See all Maleimide kits ...
- Tests1 x 200 test
- Sample typeCell culture extracts, Adherent cells, Suspension cells
- Assay typeQuantitative
- Product overview
ab112141 uses a proprietary dye that has enhanced fluorescence upon reacting with a maleimide. The kit provides a sensitive, one-step fluorometric method to detect as little as 10 picomoles of maleimide in a 100 µL assay volume (100 nM; Figure 1). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 490/520 nm. Compared to ab112140, this fluorometric assay is more sensitive, and has less interference from biological samples.
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ab112141 should be stored desiccated.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 200 tests Assay Buffer 1 x 25ml DMSO 1 x 200µl MalemGreen indicator 1 vial N-ethylmaleimide Standard 1 x 50µl Reaction Buffer 1 x 500µl
- Research Areas
Our Abpromise guarantee covers the use of ab112141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Maleimide Quantification Assay Kit (Fluorometric) images
N-ethylmaleimide dose response was measured in a 96-well black plate with ab112141 using a microplate reader. As low as 0.1 µM (10 picomol/well) of maleimide can be detected with 10 minutes incubation time (n=3).
References for Maleimide Quantification Assay Kit (Fluorometric) (ab112141)
ab112141 has not yet been referenced specifically in any publications.