Recombinant Anti-Mammaglobin A antibody [EPR9092] - Low endotoxin, Azide free (ab215384)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9092] to Mammaglobin A - Low endotoxin, Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-Mammaglobin A antibody [EPR9092] - Low endotoxin, Azide free
See all Mammaglobin A primary antibodies -
Description
Rabbit monoclonal [EPR9092] to Mammaglobin A - Low endotoxin, Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Mammaglobin A.
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General notes
ab215384 is the carrier-free version of ab150359.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9092 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab215384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. |
Target
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Tissue specificity
Mammary gland specific. Over-expressed in breast cancer. -
Sequence similarities
Belongs to the secretoglobin family. Lipophilin subfamily. - Information by UniProt
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Database links
- Entrez Gene: 4250 Human
- Omim: 605562 Human
- SwissProt: Q13296 Human
- Unigene: 46452 Human
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Alternative names
- Mammaglobin 1 antibody
- Mammaglobin-1 antibody
- Mammaglobin-A antibody
see all
Images
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This data was developed using ab150359, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue sections labeling Mammaglobin A with purified ab150359 at 1/1200 dilution (0.54 µg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. -
Anti-Mammaglobin A antibody [EPR9092] (ab150359) at 1/1000 dilution (Purified) + SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 14-21 kDa why is the actual band size different from the predicted?This data was developed using ab150359 the same antibody clone in a different buffer formulation.
The molecular weight observed is consistent with what has been described in the literature (PMID:16166429). Bands at 14-21.
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Anti-Mammaglobin A antibody [EPR9092] (ab150359) at 1/20000 dilution (Purified) + Human breast cancer lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 14-21 kDa why is the actual band size different from the predicted?This data was developed using ab150359 the same antibody clone in a different buffer formulation.
The molecular weight observed is consistent with what has been described in the literature (PMID:16166429). Bands at 14-21.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab215384 has not yet been referenced specifically in any publications.