You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Mammalian Cell Extraction Kit (ab65399)

Q&A (2)

Overview

  • Product nameMammalian Cell Extraction Kit
  • Tests
    500 x 1 assay
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay typeDirect
  • Assay time
    0h 20m
  • Product overview

    Abcam's Mammalian Cell Extraction Kit provides optimized cell extraction buffer, protease inhibitor cocktail, and DTT for convenient extraction of mammalian proteins from cultured cells and tissue samples, under nondenaturing conditions. Cell lysate prepared using the kit can be used in a variety of applications, such as enzyme activity assays (e.g. caspase activity assays), Western blot analysis, and others. The entire procedure takes less than 20 minutes.
    Visit our FAQs page for tips and troubleshooting.

Properties

  • RelevanceMammalian Cell Extraction methods allow extraction of mammalian proteins from cultured cells and tissue samples.

    • Protocols

    References for Mammalian Cell Extraction Kit (ab65399)

    ab65399 has not yet been referenced specifically in any publications.

    Publishing research using ab65399 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab65399 Submit a Question for ab65399

    Displaying 1 - 2 of 2 results for Abreviews and Q&A

    10
    Votes: Highest First

    Dear Ms or Mrs,

    Thank you very much for your patient explanation !

    May I ask some new questions about this product:

    1.We want to extract the proteins include membrane proteins and nuclear proteinsfrom rat testes, Can we us...

    Read More

    I double checked with the lab to answer this question.

    1. I am happy to confirm that this kit can be used to isolate both membrane and cytoplasmatic proteins. The membrane proteins are in the pellet from step 5. If you would like to also isolate...

    Read More

    To whom may concerns,

    Thank you for your attention.

    I am seeing the product named 'mammalian cell extraction kit',its item is ab65399fromabcam.There has no protocal about this product , I am just wondering toask some questions :

    Read More

    Thank you for your inquiry.

    Please find the protocol for this kit below:

    Mammalian Protein Extraction Protocol

    A. General Consideration and Reagent Preparation:

    - After opening the kit, store Cell Extraction Buffer ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"