Products:Immunology >> Innate Immunity >> Macrophage / Inflamm.
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I am interested in purchasing a selection of antibodies for the study of truncated variants of Bax, Caspase 5 and TGF beta receptor II. The following antibodies may be of use to me, as your datasheets indicate that the N-terminus has been used as the immunogen. However, I need to know which specific residues have been used in order to know whether the antibodies are going to be helpful. I wish to use the antibodies in Western Blot & IHC experiments on colon cancer cell lines. Bax ab15181 (ab33020, ab5714 & ab7977 all have specific immunogen peptide sequences supplied) Caspase 5 ab25899 ab40887 TGF beta receptor II ab28382 ab28383 I would also be interested in antibodies to the N-terminus of TAF1B, TCF7L2, IGF2R (ab26938 should be okay) and MSH3. Ideally I would like to order soon to have the antibodies for use by late January. Thankyou for you help with this Regards |
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ANSWER: |
Thank you for your enquiry. I have contacted the originators of these antibodies and here is the sequence information I obtained. ab25899: This immunogen sequence is proprietary, but we can run any alignments with the sequence that you require. ab40887: The immunogen is 19aa long peptide that lies within the first 50 aas on N-termincal of human Caspase-5. ab28383/ab28382:LDTLVGKGRFAEVYKAKLKQN (these are the same antibody, one is prediluted and one is concentrated) ab15181: GSGEQPRGGGPTSSC Before buying any of these antibodies, I recommend that you check to make sure they are tested in Western blot and IHC and also make sure they have been tested in the sepcies of the sample you are using. We can only guarantee the antibodies if you use them as they are described on the data sheets. I hope this information helps, please do not hesitate to contact us if you need any more advice or information. |
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