Anti-Mannose 6 Phosphate Receptor (Cation independent) antibody [MEM-238] (ab8093)

Immunocytochemistry/ Immunofluorescence - IGF2 Receptor antibody [MEM-238] (ab8093)

ab8093 at 5µg/ml staining human HEK cells by immunocytochemistry. The cells were fixed with paraformaldehyde and incubated with the antibody for 1 hour. Bound antibody was detected using a goat anti-mouse IgG Alexa-Fluor ® 568. In the confocal image ab8093 labelling in red shows a distribution consistent with the location of the trans-Golgi network and lysosomes. Blue nuclear counterstain is present.

This image is courtesy of an Abreview submitted by Randal Moldrich on 31 March 2006.

Immunocytochemistry/ Immunofluorescence - Mannose 6 Phosphate Receptor (Cation independent) antibody [MEM-238] (ab8093)

ab8093 staining Mannose 6 Phosphate Receptor (Cation independent) in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde in PBS, permeabilized with 0.1% Triton X-100 and blocked with 2% BSA for 30 minutes at 25°C . Samples were incubated with primary antibody (1/300 in 2%BSA + 0.1% Triton X-100) for 2 hours at 25°C. An Alexa Fluor^® 488-conjugated Goat polyclonal to mouse IgG (dilution 1/2000) was used as secondary antibody.

This image is courtesy of an anonymous Abreview

Flow Cytometry - Mannose 6 Phosphate Receptor (Cation independent) antibody [MEM-238] (ab8093)

Overlay histogram showing HeLa cells stained with ab8093 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8093, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.