Anti-Mannose 6 Phosphate Receptor (Cation independent) antibody (ab88579)

Overview

• Product nameAnti-Mannose 6 Phosphate Receptor (Cation independent) antibodySee all Mannose 6 Phosphate Receptor (Cation independent) primary antibodies ...
• Description
  Rabbit polyclonal to Mannose 6 Phosphate Receptor (Cation independent)
• Tested applicationsICC/IF, WB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Rabbit, Dog
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 1550 - 1650 of Human Mannose 6 Phosphate Receptor (Cation independent).

  Read Abcam's proprietary immunogen policy

• Positive controlThis antibody gave a positive signal in HepG2 and U2Os whole cell lysates.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Trafficking
  • Golgi Proteins

  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Endosome

  • Immunology
  • Innate Immunity
  • Macrophage / Inflamm.

Applications

Target

• FunctionTransport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.
• Sequence similaritiesBelongs to the MRL1/IGF2R family.
  Contains 1 fibronectin type-II domain.
• DomainContains 15 repeating units of approximately 147 AA. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends.
• Cellular localizationLysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface.

Target information above from: UniProt accession P11717 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3482 Human
  • Omim: 147280 Human
  • SwissProt: P11717 Human
  • Unigene: 487062 Human

• Alternative names
  300 kDa mannose 6 phosphate receptor antibody300 kDa mannose 6-phosphate receptor antibodyCation independent mannose 6 phosphate receptor antibody

  Cation-independent mannose-6-phosphate receptor antibodyCD222 antibodyCD222 antigen antibodyCI Man 6 P receptor antibodyCI Man-6-P receptor antibodyCI MPR antibodyCI-MPR antibodyCIMPR antibodyIGF 2 receptor antibodyIGF 2R antibodyIGF II receptor antibodyIGF-II receptor antibodyIGF2 receptor antibodyIGF2R antibodyInsulin like growth factor 2 receptor antibodyInsulin like growth factor II receptor antibodyInsulin-like growth factor 2 receptor antibodyInsulin-like growth factor II receptor antibodyM6P R antibodyM6P/IGF2 receptor antibodyM6P/IGF2R antibodyM6PR antibodyMPR 300 antibodyMPR300 antibodyMPRI antibodyMPRI_HUMAN antibody

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Anti-Mannose 6 Phosphate Receptor (Cation independent) antibody images

• 

  Western blot - Mannose 6 Phosphate Receptor (Cation independent) antibody (ab88579)
  All lanes : Anti-Mannose 6 Phosphate Receptor (Cation independent) antibody (ab88579) at 1 µg/ml
  
  Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
  Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 274 kDa
  Observed band size : 274 kDa
  Additional bands at : 35 kDa,45 kDa,50 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 12 minutes
• 

  Immunocytochemistry/ Immunofluorescence - Mannose 6 Phosphate Receptor (Cation independent) antibody (ab88579)
  ICC/IF image of ab88579 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88579, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.