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Overview
Product name
Anti-Matrin 3 antibody
See all Matrin 3 products (5) ...
Description
Rabbit polyclonal to Matrin 3
Tested applications
IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide designed within residues: GFYCKLCSLF YTNEEVAKNT HCSSLPHYQK LKKFLNKLAE ERRQKKET, corresponding to C terminal amino acids 800-847 of Human Matrin 3 (NP_061322.2)
G FYCKLCSLFY TNEEVAKNTH CSSLPHYQKL KKFLN KLAEERRQKK ET
Positive control
Human: breast carcinoma, colon carcinoma, laryngeal squamous cell carcinoma, linitis plastica stomach cancer, lung adenocarcinoma, metastatic lymph node, non-small cell lung cancer, ovarian carcinoma, pancreatic islet cell tumor, prostate carcinoma, skin basal cell carcinoma, skin squamous cell carcinoma, small cell lung cancer, stomach adenocarcinoma, testicular seminoma. Mouse: squamous cell carcinoma.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab84422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/500.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.(Note: expected to work on frozen sections.)
ICC/IF: 1/100
Target
Function
May play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. In association with the SFPQ-NONO heteromer may play a role in nuclear retention of defective RNAs.
Involvement in disease
Defects in MATR3 are the cause of myopathy distal type 2 (MPD2) [MIM:606070]; also called vocal cord and pharyngeal dysfunction with distal myopathy (VCPDM). MPD2 is a muscular disorder characterized by distal weakness, with onset in hands and feet, associated with vocal cord and pharyngeal weakness causing a nasal voice and swallowing disorders.
Sequence similarities
Contains 1 matrin-type zinc finger.
Contains 2 RRM (RNA recognition motif) domains.
Cellular localization
Nucleus matrix.
Target information above from: UniProt accessionP43243
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MATR3 antibody
- MATR3_HUMAN antibody
- Matrin-3 antibody
- Matrin3 antibody
Database links
- Entrez Gene: 9782 Human
- Entrez Gene: 17184 Mouse
- Omim: 164015 Human
- SwissProt: P43243 Human
- SwissProt: Q3TTX0 Mouse
- SwissProt: Q4FJT0 Mouse
- SwissProt: Q5BL18 Mouse
- SwissProt: Q8K310 Mouse
see all
Anti-Matrin 3 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Matrin 3 antibody (ab84422)
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human colon adenocarcinoma using 1/250 ab84422, followed by DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Matrin 3 antibody (ab84422)
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Mouse squamous cell carcinoma using 1/250 ab84422, followed by DAB staining.
Immunocytochemistry/ Immunofluorescence - Matrin 3 antibody (ab84422)
Detection of Human Matrin 3 by Immunofluorescence.Sample: FFPE section of human breast carcinoma.Antibody: ab84422 used at a dilution of 1/100.Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG used at a dilution of 1/500.
Immunocytochemistry/ Immunofluorescence - Matrin 3 antibody (ab84422)
Detection of Human Matrin 3 by Immunofluorescence.Sample: FFPE section of Human breast carcinoma.Antibody: ab84422 used at a dilution of 1/100.Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG used at a dilution of 1/500.
Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody (ab84422)
ab84422 staining Matrin 3 in human U2OS cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0.5% NP-40, blocked with 1% BSA for 1 hour a room temperature, then incubated with ab84422 at a 1/100 dilution for 1 hour. The secondary antibody used was an Alexa-Fluor 488 conjugate donkey polyclonal, used at a 1/1000 dilution.
Image courtesy of Leilani Beltran by Abreview.
References for Anti-Matrin 3 antibody (ab84422)
ab84422 has not yet been referenced specifically in any publications.
Publishing research using ab84422? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"