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Anti-Matriptase 2 antibody - Catalytic domain (ab42463)

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This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab42463 for help.

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2 questions for ab42463

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Question 1

Tuesday 24-June-2008

I have noted that the WB using ab49783 and ab42463 look quite different. Whiule it is indicated that the cell ty ped probed woth ab59783 is SK-HEP-1, the source of protein for the WB with ab 43463 is not given. Could you precise and comment about the differences between the 2 Ab?

ANSWER:

  

Thank you for your enquiry and your interest in our products.

The sample used in the ab42463 Western blot image (correctly noted on the Abcam website) is a dilution of human milk. The epitope for ab42463 is towards the carboxyterminal end of the catalytic domain, and the epitope for ab59783 is closer to the aminoterminal end of the catalytic domain.

I hope this information will be useful for you.

Question 2

Thursday 28-February-2008

I would like to know the difference between ab42463 and ab59783. I need a good antibody against matriptase 2 and matriptase-1. I want to look into lysat the presence of these proteins. Which antidobies do you recommend?

ANSWER:

  

Thank you for your enquiry.

The difference between ab42463 and ab59783 is that the immunogen sequence is difference. Both immunogens are within the catalytic domain (amino acids 568- 802 of the 802 amino acid human Matriptase-2 sequence). The ab59783 epitope falls within the range of amino acids 610 - 690, and the ab42463 epitope falls within the range of amino acids 760 - 802; we cannot give out the exact amino acid sequence of any of our immunogenic peptides, because that information is proprietary. Both antibodies work well in western blotting.

It seems that we currently do not have antibodies to matriptase-1 I'm afraid.

I hope the above information is useful, please do not hesitate to contact us if you need further information.

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