Matriptase 3 antibody - Catalytic domain (ab42462)

Product Name 

Matriptase 3 antibody - Catalytic domain

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Matriptase 3 - Catalytic domain

Immunogen 

Synthetic peptide based on the catalytic domain of Human Matriptase 3.

(Peptide available as ab44751.)

Reacts with 
(species key)

Hu, Ms, Rat, Chmp, Dog

Specificity 

Reacts with Matriptase 3; does not recognize matriptase 1 or matriptase 2.

We have a range of domain specific antibodies for this target. For a full list please see all Matriptase 3 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 - 1/5000. 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. Detects a band of approximately 90 kDa (predicted molecular weight: 82 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Membrane; single-pass type II membrane protein

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> TMPRSS
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Matriptase
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Other Enzymes

Relevance 

Matriptase 3 is a Type-II serine proteinase, meaning it contains a transmembrane motif in the amino end of the protein. The extracellular domains contain a stem region, followed by a SEA domain, two CUB domains and three repeats of LDL-receptor-like homology, and a chymotrypsin-like serine protease domain located in the carboxyterminal end of the protein. Matriptase 3 is a member of the S1A family of the PA clan of serine proteinases, which includes acrosin, kallikreins, trypsin, chymotrypsin, and many other important enzymes. Matriptase 3 shares 31% sequence identity with matriptase 1 and matriptase 2. The canonical activation cleavage site of the transmembrane serine proteases is conserved in matriptase 3, and the zymogen is cleaved at R493-IIGG to produce the active enzyme. In humans and in the mouse, matriptase 3 is found in the brain, eye, testis, epididymus and salivary gland, with lower levels found in the heart, skeletal muscles, thymus, ovary and prostate. The endogenous inhibitor of matriptase 3 is thought to be HAI-1 (Hepatocyte Activator Inhibitor-1), a serine proteinase inhibitor that was determined to block the activation of HGF. Alpha-2 macroglobulin has also been shown to bind and inhibit matriptase 3 in-vitro.
Similar to other transmembrane serine proteinases, several different splice variants have been identified, all with different aminoterminal regions. The different cytoplasmic domains are thought to give specificity in regulation for matriptase 3. The three forms identified to date include 731, 706 and 572 amino acid forms. The longest human matriptase 3 sequence codes for a protein of 731 amino acids, with a predicted mass of 81.74 kDa and a pI of 8.88. The matriptase 3 protein is glycosylated, and runs with an apparent molecular weight of approximately 90 kDa. The 706 amino acid version has a predicted mass of 78.7 kDa, with pI of 8.0, and the 572 amino acid form is 64.04 kDa and 9.08 pI. Interestingly, the human sequences are known to date are all shorter than the mouse, rat, dog and chimp sequences, and start after the putative transmembrane domain. It may be that the basic pI of the human matriptase 3 sequences allows it to dock to the ECM via HS-Gag interactions, since it is known that matriptase 3 associates with the plasma membrane, but more needs to be done to confirm this speculation.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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