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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Other Enzymes
Overview
Product name
Matriptase 3 peptide (Aminotermial end long form)
See all Matriptase 3 products (4) ...
Protein description
Synthetic peptide Matriptase 3. (Note: the amino acid sequence is proprietary) This peptide was used as an immunogen for ab42461 - Matriptase 3 antibody.
Properties
Purity
>95% by SDS-PAGE
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 0.001% Tween 20, 30mM HEPES, 2mM EDTA, 150mM Sodium chloride, pH 6.75
Concentration
Concentration information loading...
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> TMPRSS
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Matriptase
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Other Enzymes
Applications
Show applications key
Our Abpromise guarantee covers the use of ab44747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This peptide can be used with studies using ab42461. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Protein info
Relevance
Matriptase 3 is a Type-II serine proteinase, meaning it contains a transmembrane motif in the amino end of the protein. The extracellular domains contain a stem region, followed by a SEA domain, two CUB domains and three repeats of LDL-receptor-like homology, and a chymotrypsin-like serine protease domain located in the carboxyterminal end of the protein. Matriptase 3 is a member of the S1A family of the PA clan of serine proteinases, which includes acrosin, kallikreins, trypsin, chymotrypsin, and many other important enzymes. Matriptase 3 shares 31% sequence identity with matriptase 1 and matriptase 2. The canonical activation cleavage site of the transmembrane serine proteases is conserved in matriptase 3, and the zymogen is cleaved at R493-IIGG to produce the active enzyme. In humans and in the mouse, matriptase 3 is found in the brain, eye, testis, epididymus and salivary gland, with lower levels found in the heart, skeletal muscles, thymus, ovary and prostate. The endogenous inhibitor of matriptase 3 is thought to be HAI-1 (Hepatocyte Activator Inhibitor-1), a serine proteinase inhibitor that was determined to block the activation of HGF. Alpha-2 macroglobulin has also been shown to bind and inhibit matriptase 3 in-vitro. Similar to other transmembrane serine proteinases, several different splice variants have been identified, all with different aminoterminal regions. The different cytoplasmic domains are thought to give specificity in regulation for matriptase 3. The three forms identified to date include 731, 706 and 572 amino acid forms. The longest human matriptase 3 sequence codes for a protein of 731 amino acids, with a predicted mass of 81.74 kDa and a pI of 8.88. The matriptase 3 protein is glycosylated, and runs with an apparent molecular weight of approximately 90 kDa. The 706 amino acid version has a predicted mass of 78.7 kDa, with pI of 8.0, and the 572 amino acid form is 64.04 kDa and 9.08 pI. Interestingly, the human sequences are known to date are all shorter than the mouse, rat, dog and chimp sequences, and start after the putative transmembrane domain. It may be that the basic pI of the human matriptase 3 sequences allows it to dock to the ECM via HS-Gag interactions, since it is known that matriptase 3 associates with the plasma membrane, but more needs to be done to confirm this speculation.
Cellular localization
Membrane; single-pass type II membrane protein
References for Matriptase 3 peptide (Aminotermial end long form) (ab44747)
ab44747 has not yet been referenced specifically in any publications.
Publishing research using ab44747? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"