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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab785 for help.
There are no answered questions for ab785
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![- MelanA antibody [A103] (ab785)](/ps/datasheet/Images/0/ab785/ab785_1.jpg)
- MelanA antibody [A103] (ab785)
ab785 - immunohistochemistry
![Flow Cytometry - Anti-MelanA antibody [A103] (ab785)](/ps/datasheet/images/0/ab785/MelanA-Primary-antibodies-ab785-2.jpg)
Flow Cytometry - Anti-MelanA antibody [A103] (ab785)
Overlay histogram showing Malme-3 cells stained with ab785 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab785, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [A103] (ab785)](/ps/datasheet/images/0/ab785/MelanA-Primary-antibodies-ab785-3.jpeg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [A103] (ab785)
IHC image of ab785 staining in Human Skin Melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab785, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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