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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Overview
Product name
Anti-MelanA antibody [A103], prediluted
See all MelanA products (12) ...
Description
Mouse monoclonal [A103] to MelanA, prediluted
Specificity
No cross-reactivity to MAGE 1 and Tyrosinase. This antibody reacts positively with angiomyolipoma and steroid hormone producing cells of the adrenal gland, ovary, testis and especially with carcinoma of these organs.
Tested applications
IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein
Positive control
Melanoma
Properties
Form
Prediluted
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: Stabilizing phosphate buffer, pH 7.3
Concentration
Concentration information loading...
Primary antibody notes
This antibody is suitable for the detection of MelanA antigen in cells of melanoic origin. The antibody will label 80-90% of metastasising malign melanoma. MelanA negative melanoma usually are of the spindle cell or desmoblastic type.
Clonality
Monoclonal
Clone number
A103
Isotype
IgG1
Research areas
Cancer >> Tumor immunology >> Tumor-associated antigens
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
Application notes
IHC-P: Use 100 µl working solution / test. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
Tissue specificity
Expression is restricted to melanoma and melanocyte cell lines and retina.
Post-translational
modifications
Acylated.
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus. Golgi apparatus > trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.
Target information above from: UniProt accessionQ16655
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Antigen LB39 AA antibody
- Antigen LB39-AA antibody
- Antigen SK29 AA antibody
see all
Database links
- Entrez Gene: 2315 Human
- Omim: 605513 Human
- SwissProt: Q16655 Human
- Unigene: 154069 Human
References for Anti-MelanA antibody [A103], prediluted (ab51936)
ab51936 has not yet been referenced specifically in any publications.
Publishing research using ab51936? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"