MelanA antibody [DT101 + BC199 ] (ab731)

Product Name 

MelanA antibody [DT101 + BC199 ]

Product type 

Primary antibodies

Description 

Mouse monoclonal [DT101 + BC199 ] to MelanA

Immunogen 

Recombinant full length protein (Human).

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

This antibody recognizes a protein of 18kDa, identified at MART-1 (Melanoma Antigen Recognized by T- cells 1) or Melan-A. The MART-1 recognizes a subcellular fraction found in melanosomes. The antibody labels melanomas and tumors showing melanocytic differentiation. It does not mark neoplasms of epithelial origin, lymphomas or mesenchymal tumors. Melan-A is a useful addition to melanoma panels as it is apparently specific for melanocytic lesions. Both HMB-45 and MART-1 are coexpressed in the majority of melanomas, as well as uniquely expressed in certain cases. Studies have shown that MART-1 is more sensitive than HMB-45 when labeling metastatic melanomas.

Tested applications 
(see key)

IHC-Fr, IHC-P

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
IHC:
For an ABC system, dilute 1:50-100 and incubate for 30-60 minutes at RT.
For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval before commencing with IHC.
It is sometimes difficult to interpret DAB stained melanomas due to endogenous pigment.
We recommend you substitute an AEC or a Fast Red substrate protocol.
An avidin biotin blocking may be required for certain types of melanomas.

Positive control 
(see definition)

Melanoma

Cellular localization 

Membrane; Single pass membrane protein

Research areas 

Cancer >> Tumor immunology >> Tumor-associated antigens
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated

Relevance 

MelanA or MART1 (Melanoma Antigen Recognized by T cells) or is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE1, MAGE3, tyrosinase, gp100, gp75, BAGE1, and GAGE1.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

DT101 + BC199

Isotype 

IgG

Storage buffer 

Preservative: Proprietary, not sodium azide or thimerosal.
Constituents: PBS, Tissue Culture Supernatant. pH 7.3; Protein carrier.

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C.

Notes 

This antibody is a mixture of two clones, DT101 and BC199.

References for MelanA antibody [DT101 + BC199 ] (ab731)

  This product has been used in: (two most recent references)

Blessing K et al. Comparison of immunohistochemical staining of the novel antibody melan-A with S100 protein and HMB-45 in malignant melanoma and melanoma variants. Histopathology 32:139-46 (1998). PubMed: 9543670

Kageshita T et al. Differential expression of MART-1 in primary and metastatic melanoma lesions. J Immunother 20:460-5 (1997). PubMed: 9409451
If you publish research using ab731 please let us know so that we can cite the reference on this datasheet.

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