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Anti-MelanA antibody [DT101 + BC199] (ab731)

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Overview

Product name

Anti-MelanA antibody [DT101 + BC199]
See all MelanA products (12) ...

Description

Mouse monoclonal [DT101 + BC199] to MelanA

Specificity

This antibody recognizes a protein of 18kDa, identified at MART-1 (Melanoma Antigen Recognized by T- cells 1) or Melan-A. The MART-1 recognizes a subcellular fraction found in melanosomes. The antibody labels melanomas and tumors showing melanocytic differentiation. It does not mark neoplasms of epithelial origin, lymphomas or mesenchymal tumors. Melan-A is a useful addition to melanoma panels as it is apparently specific for melanocytic lesions. Both HMB-45 and MART-1 are coexpressed in the majority of melanomas, as well as uniquely expressed in certain cases. Studies have shown that MART-1 is more sensitive than HMB-45 when labeling metastatic melanomas.

Tested applications

IHC-P, IHC-Frmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Recombinant full length protein (Human).

Positive control

Melanoma

General notes

This antibody is a mixture of two clones, DT101 and BC199.

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: Proprietary, not sodium azide or thimerosal.
Constituents: PBS, Tissue Culture Supernatant. pH 7.3; Protein carrier.

Concentration

Concentration information loading...

Clonality

Monoclonal

Clone number

DT101 + BC199

Myeloma

unknown

Isotype

IgG

Light chain type

unknown

Research areas

Cancer >> Tumor immunology >> Tumor-associated antigens
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated

Applications

Show applications key

Our Abpromise guarantee covers the use of ab731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    IHC-P

    Five stars (1 Abreview) IHC-P: 1/50 - 1/100.Perf...Read more →

    IHC-P: 1/50 - 1/100.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.(ABC method.)

  • ShowHide

    IHC-Fr

      See more...Read more →

    IHC-Fr: 1/50 - 1/100.(ABC method.

    It is sometimes difficult to interpret DAB stained melanomas due to endogenous pigment. We recommend you substitute an AEC or a Fast Red substrate protocol. An avidin biotin blocking may be required for certain types of melanomas. )

Target

Function

Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.

Tissue specificity

Expression is restricted to melanoma and melanocyte cell lines and retina.

Post-translational
modifications

Acylated.

Cellular localization

Endoplasmic reticulum membrane. Golgi apparatus. Golgi apparatus > trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.

Target information above from: UniProt accessionQ16655 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Antigen LB39 AA antibody
  • Antigen LB39-AA antibody
  • Antigen SK29 AA antibody
  • Antigen SK29-AA antibody
  • MAR1_HUMAN antibody
  • MART 1 antibody
  • MART-1 antibody
  • MART1 antibody
  • Melan A antibody
  • Melan A protein antibody
  • Melanoma antigen recognized by T cells 1 antibody
  • Melanoma antigen recognized by T-cells 1 antibody
  • MLAN A antibody
  • MLANA antibody
  • OTTHUMP00000021036 antibody
  • OTTHUMP00000021037 antibody
  • OTTHUMP00000021038 antibody
  • Protein Melan-A antibody
see all

Database links

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References for Anti-MelanA antibody [DT101 + BC199] (ab731)

This product has been referenced in:

  • Blessing Ket al. Comparison of immunohistochemical staining of the novel antibody melan-A with S100 protein and HMB-45 in malignant melanoma and melanoma variants. Histopathology 32:139-46 (1998).Read more (PubMed: 9543670) »
  • Kageshita Tet al. Differential expression of MART-1 in primary and metastatic melanoma lesions. J Immunother 20:460-5 (1997).Read more (PubMed: 9409451) »

See all 2 publications for this product

Publishing research using ab731? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"