![Western blot - MelanA antibody [EP1422Y] (ab51061) image](#ab51061_1.jpg)
![Immunohistochemistry (Paraffin-embedded sections) - MelanA antibody [EP1422Y] (ab51061) image](#ab51061_2.jpg)
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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Overview
Product name
Anti-MelanA antibody [EP1422Y]
See all MelanA products (12) ...
Description
Rabbit monoclonal [EP1422Y] to MelanA
Tested applications
WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
Synthetic peptide corresponding to residues on the N-terminus of human MelanA.
Positive control
Melanoma lysate or melanoma tissue.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP1422Y
Isotype
IgG
Research areas
Cancer >> Tumor immunology >> Tumor-associated antigens
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Detects a band of approximately 20 kDa (predicted molecular weight: 13 kDa).
IHC-P: 1/100 - 1/250.(We strongly recommend that customers perform an antigen retrieval step.)
IHC-Fr: Use at an assay dependent concentration.
Application notes
Is unsuitable for ,Flow Cyt or IP.
Target
Function
Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
Tissue specificity
Expression is restricted to melanoma and melanocyte cell lines and retina.
Post-translational
modifications
Acylated.
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus. Golgi apparatus > trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.
Target information above from: UniProt accessionQ16655
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Antigen LB39 AA antibody
- Antigen LB39-AA antibody
- Antigen SK29 AA antibody
see all
Database links
- Entrez Gene: 2315 Human
- Omim: 605513 Human
- SwissProt: Q16655 Human
- Unigene: 154069 Human
Anti-MelanA antibody [EP1422Y] images:
Western blot - MelanA antibody [EP1422Y] (ab51061)
![Western blot - MelanA antibody [EP1422Y] (ab51061)](/ps/datasheet/Images/51/ab51061/ab51061_1.jpg)
Anti-MelanA antibody [EP1422Y] (ab51061) at 1/1000 dilution + Melanoma lysate at 10 µg
Secondary
Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size : 13 kDa
Observed band size : 20 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - MelanA antibody [EP1422Y] (ab51061)
![Immunohistochemistry (Paraffin-embedded sections) - MelanA antibody [EP1422Y] (ab51061)](/ps/datasheet/Images/51/ab51061/ab51061_2.jpg)
Ab51061 (1/100) staining human MelanA in melanoma tissue by immunohistochemistry using paraffin embedded tissue.
References for Anti-MelanA antibody [EP1422Y] (ab51061)
ab51061 has not yet been referenced specifically in any publications.
Publishing research using ab51061? Please let us know so that we can cite the reference in this datasheet
![Anti-MelanA antibody [EP1422Y] for Immunohistochemistry (Frozen sections) in Human (51061)](/ps/datasheet/images/51/ab51061/MelanA-Primary-antibodies-ab51061-4.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"