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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab3168 for help.
There are no answered questions for ab3168
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - MelanA antibody [M2-7C10] (ab3168)](/ps/datasheet/images/3/ab3168/MelanA-Primary-antibodies-ab3168-2.jpg)
Western blot - MelanA antibody [M2-7C10] (ab3168)
All lanes : Anti-MelanA antibody [M2-7C10] (ab3168) at 1 µg/ml
Lane 1 : WERI (Human Retinoblastoma) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 13 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa,65 kDa,74 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
The predicted molecular weight of is 13 kDa (SwissProt), however we expect to observe a banding pattern at 18 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
![Flow Cytometry - MelanA antibody [M2-7C10] (ab3168)](/ps/datasheet/images/3/ab3168/MelanA-Primary-antibodies-ab3168-3.jpg)
Flow Cytometry - MelanA antibody [M2-7C10] (ab3168)
Overlay histogram showing Malme-3 cells stained with ab3168 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3168, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
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