![Western blot - MelanA antibody [M2-7C10] (ab3168) image](#MelanA-Primary-antibodies-ab3168-2.jpg)
![Flow Cytometry - MelanA antibody [M2-7C10] (ab3168) image](#MelanA-Primary-antibodies-ab3168-3.jpg)
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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Overview
Product name
Anti-MelanA antibody [M2-7C10]
See all MelanA products (12) ...
Description
Mouse monoclonal [M2-7C10] to MelanA
Specificity
ab3168 labels melanomas and other tumors showing melanocytic differentiation. It does not stain tumor cells of epithelial, lymphoid, glial, ormesenchymal origin.
Tested applications
ICC/IF, Flow Cyt, IHC-P, IP, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Horse
Does not react with
Mouse, Rat
Immunogen
Recombinant hMART-1 protein.
Positive control
CaC1 melanoma cells and melanomas.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
M2-7C10
Isotype
IgG2b
Light chain type
kappa
Research areas
Cancer >> Tumor immunology >> Tumor-associated antigens
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3168 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent concentration. (PubMed: 21730137)
Flow Cyt: Use 2µg for 106 cells.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: Use at an assay dependent dilution. ((denatured). Use at a concentration of 2µg / mg.)
WB: Use a concentration of 0.5 - 1 µg/ml.Detects a band of approximately 18 kDa (predicted molecular weight: 13 kDa).
Target
Function
Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
Tissue specificity
Expression is restricted to melanoma and melanocyte cell lines and retina.
Post-translational
modifications
Acylated.
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus. Golgi apparatus > trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.
Target information above from: UniProt accessionQ16655
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Antigen LB39 AA antibody
- Antigen LB39-AA antibody
- Antigen SK29 AA antibody
see all
Database links
- Entrez Gene: 2315 Human
- Omim: 605513 Human
- SwissProt: Q16655 Human
- Unigene: 154069 Human
Anti-MelanA antibody [M2-7C10] images:
Western blot - MelanA antibody [M2-7C10] (ab3168)
![Western blot - MelanA antibody [M2-7C10] (ab3168)](/ps/datasheet/images/3/ab3168/MelanA-Primary-antibodies-ab3168-2.jpg)
All lanes : Anti-MelanA antibody [M2-7C10] (ab3168) at 1 µg/ml
Lane 1 : WERI (Human Retinoblastoma) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 13 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa,65 kDa,74 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
The predicted molecular weight of is 13 kDa (SwissProt), however we expect to observe a banding pattern at 18 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Flow Cytometry - MelanA antibody [M2-7C10] (ab3168)
![Flow Cytometry - MelanA antibody [M2-7C10] (ab3168)](/ps/datasheet/images/3/ab3168/MelanA-Primary-antibodies-ab3168-3.jpg)
Overlay histogram showing Malme-3 cells stained with ab3168 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3168, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in Malme-3 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
References for Anti-MelanA antibody [M2-7C10] (ab3168)
This product has been referenced in:
- Giordano Fet al. The ocular albinism type 1 (OA1) GPCR is ubiquitinated and its traffic requires endosomal sorting complex responsible for transport (ESCRT) function. Proc Natl Acad Sci U S A 108:11906-11 (2011). ICC/IF; Human.Read more (PubMed: 21730137) »
- Pietra Get al. Natural killer cells kill human melanoma cells with characteristics of cancer stem cells. Int Immunol 21:793-801 (2009).Read more (PubMed: 19491215) »
See all 2 publications for this product
Publishing research using ab3168? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"