|
|
|
|
|
Product Name
|
MelanA antibody [M2-7C10]
|
| |
See all MelanA antibodies (9)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [M2-7C10] to MelanA
|
|
Immunogen
|
Recombinant hMART-1 protein.
|
|
Reacts with
|
Hu, Hrs
|
|
Does not react with
(species key)
|
Ms, Rat
|
|
Specificity
|
ab3168 labels melanomas and other tumors showing melanocytic differentiation. It does not stain tumor cells of epithelial, lymphoid, glial, ormesenchymal origin.
|
|
Tested applications
(see key)
|
Flow Cyt, IHC-P, IP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
Flow Cytometry: assay dependent.
Immunoprecipitation (denatured): use at a concentration of 2µg / mg.
Western Blotting: use at a concentration of 0.5 - 1µg / ml for 2hrs at RT.
Immunohistochemistry (formalin fixed paraffin embedded): 1 - 2µg / ml for 30 min at RT.
By Western blot, this antibody detects a band of 18 kDa, which corresponds to the predicted molecular weight of MART-1.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
|
|
Positive control
(see definition)
|
CaC1 melanoma cells and melanomas.
|
|
Cellular localization
|
Membrane; Single pass membrane protein
|
|
Research areas
|
Cancer >> Tumor immunology >> Tumor-associated antigens
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
|
|
Relevance
|
MelanA or MART1 (Melanoma Antigen Recognized by T cells) or is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE1, MAGE3, tyrosinase, gp100, gp75, BAGE1, and GAGE1.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
M2-7C10
|
|
Isotype
|
IgG2b
|
|
Light chain type
|
kappa
|
|
Purity
|
Protein A purified
|
|
Storage buffer
|
10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
0.200 mg/ml
|
|
Storage instructions
|
Store at +4°C. Do not freeze.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MelanA antibody [M2-7C10] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
References for MelanA antibody [M2-7C10] (ab3168)
|
|
This product has been used in:
Pietra G et al. Natural killer cells kill human melanoma cells with characteristics of cancer stem cells. Int Immunol 21:793-801 (2009). PubMed: 19491215
If you publish research using ab3168 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to MelanA
|
| |
MelanA antibody [M2-7C10] - more information
|
Customer reviews (feedback) regarding MelanA antibody [M2-7C10] |
Customer FAQs regarding MelanA antibody [M2-7C10] |
Protocols for MelanA antibody [M2-7C10] |
Price and availability of products related to MelanA antibody [M2-7C10] |
| |
MelanA antibody [M2-7C10] - related products:
|
|
|
| |
MelanA antibody [M2-7C10] - other tools:
|
Contact Abcam with a Technical Enquiry about MelanA antibody [M2-7C10] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
