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Product Name 

MelanA antibody [M2-7C10]

 

See all MelanA antibodies (9)...

Product type 

Primary antibodies

Description 

Mouse monoclonal [M2-7C10] to MelanA

Immunogen 

Recombinant hMART-1 protein.

Reacts with 

Hu, Hrs

Does not react with 
(species key)

Ms, Rat

Specificity 

ab3168 labels melanomas and other tumors showing melanocytic differentiation. It does not stain tumor cells of epithelial, lymphoid, glial, ormesenchymal origin.

Tested applications 
(see key)

Flow Cyt, IHC-P, IP, WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
Flow Cytometry: assay dependent.
Immunoprecipitation (denatured): use at a concentration of 2µg / mg.
Western Blotting: use at a concentration of 0.5 - 1µg / ml for 2hrs at RT.
Immunohistochemistry (formalin fixed paraffin embedded): 1 - 2µg / ml for 30 min at RT.

By Western blot, this antibody detects a band of 18 kDa, which corresponds to the predicted molecular weight of MART-1.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Positive control 
(see definition)

CaC1 melanoma cells and melanomas.

Cellular localization 

Membrane; Single pass membrane protein

Research areas 

Cancer >> Tumor immunology >> Tumor-associated antigens
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated

Relevance 

MelanA or MART1 (Melanoma Antigen Recognized by T cells) or is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE1, MAGE3, tyrosinase, gp100, gp75, BAGE1, and GAGE1.

 

Alternative names MelanA antibody [M2-7C10] (ab3168)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

2315    (Human)

GeneCard

    

GC09P005872    (Human)

Omim

    

605513    (Human)

SwissProt

    

Q16655    (Human)

Unigene

    

154069    (Human)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

M2-7C10

Isotype 

IgG2b

Light chain type 

kappa

Purity 

Protein A purified

Storage buffer 

10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MelanA antibody [M2-7C10] is on this datasheet. But please do contact us if you would like any reassurance!



References for MelanA antibody [M2-7C10] (ab3168)

Specific publication references   This product has been used in:

Pietra G et al. Natural killer cells kill human melanoma cells with characteristics of cancer stem cells. Int Immunol 21:793-801 (2009). PubMed: 19491215

If you publish research using ab3168 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to MelanA

 

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MelanA antibody [M2-7C10] - related products:

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