Anti-Melanoma gp100 antibody (ab116582)

Overview

• Product nameAnti-Melanoma gp100 antibodySee all Melanoma gp100 primary antibodies ...
• Description
  Rabbit polyclonal to Melanoma gp100
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 411-440 of Human Melanoma gp100 (NP_008859.1).

• Positive controlCEM cell line lysate.

Properties

• FormLiquid
• Storage instructionsStore at 4°C (up to 6 months). For long term storage store at -20°C
• Storage bufferPreservative: 0.09% Sodium azide
  Constituent: 99% PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab116582 is purified through a Protein A column, followed by peptide affinity purification.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Amino acid metabolism

  • Cancer
  • Tumor immunology
  • Tumor-associated antigens

  • Signal Transduction
  • Metabolism
  • Amino Acids

  • Tags & Cell Markers
  • Cell Type Markers
  • Tumor Associated

Applications

Target

• FunctionPlays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.
• Tissue specificityPreferentially expressed in melanomas. Some expression was found in dysplastic nevi. Not found in normal tissues nor in carcinomas. Normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth.
• Sequence similaritiesBelongs to the PMEL/NMB family.
  Contains 1 PKD domain.
• DomainThe RPT domain is essential for the generation of the fibrillar matrix of melanosomes.
  The lumenal domain is necessary for correct processing and trafficking to melanosomes.
• Post-translational
  modificationsA small amount of P1/P100 (major form) undergoes glycosylation to yield P2/P120 (minor form). P2 is cleaved by a furin-like proprotein convertase (PC) in a pH-dependent manner in a post-Golgi, prelysosomal compartment into two disulfide-linked subunits: a large lumenal subunit, M-alpha/ME20-S, and an integral membrane subunit, M-beta. Despite cleavage, only a small fraction of M-alpha is secreted, whereas most M-alpha and M-beta remain associated with each other intracellularly. M-alpha is further processed to M-alpha N and M-alpha C. M-alpha C further undergoes processing to yield M-alpha C1 and M-alpha C3 (M-alpha C2 in the case of PMEL17-is or PMEL17-ls). Formation of intralumenal fibrils in the melanosomes requires the formation of M-alpha that becomes incorporated into the fibrils. Stage II melanosomes harbor only Golgi-modified Pmel17 fragments that are derived from M-alpha and that bear sialylated O-linked oligosaccharides.
  N-glycosylated. O-glycosylated; contains sialic acid.
• Cellular localizationSecreted and Endoplasmic reticulum membrane. Golgi apparatus. Melanosome. Endosome > multivesicular body. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes predominantly to intralumenal vesicles (ILVs) within multivesicular bodies. Associates with ILVs found within the lumen of premelanosomes and melanosomes and particularly in compartments that serve as precursors to the striated stage II premelanosomes.

Target information above from: UniProt accession P40967 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 6490 Human
  • Omim: 155550 Human
  • SwissProt: P40967 Human
  • Unigene: 95972 Human

• Alternative names
  95 kDa melanocyte specific secreted glycoprotein antibody95 kDa melanocyte-specific secreted glycoprotein antibodyD12S53E antibody

  gp100 antibodyM-beta antibodyME20 antibodyME20 M/ME20 S antibodyME20-M antibodyME20-S antibodyME20M antibodyME20M/ME20S antibodyME20S antibodyMelanocyte lineage specific antigen GP100 antibodyMelanocyte protein mel 17 antibodyMelanocyte protein Pmel 17 antibodyMelanocyte protein Pmel 17 precursor antibodyMelanocytes lineage-specific antigen GP100 antibodyMelanoma associated ME20 antigen antibodyMelanoma gp100 antibodyMelanoma-associated ME20 antigen antibodyMelanosomal matrix protein 17 antibodyMelanosomal matrix protein17 antibodyP1 antibodyp100 antibodyp26 antibodyPMEL 17 antibodyPMEL antibodyPMEL_HUMAN antibodyPMEL17 antibodyPremelanosome protein antibodySecreted melanoma-associated ME20 antigen antibodySI antibodySIL antibodySILV antibodySilver (mouse homolog) like antibodySilver homolog antibodySilver locus protein homolog antibodySilver, mouse, homolog of antibody

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Anti-Melanoma gp100 antibody images

• 

  Western blot - Anti-Melanoma gp100 antibody (ab116582)
  Anti-Melanoma gp100 antibody (ab116582) at 1/100 dilution + CEM cell line lysate at 35 µg
  
  Predicted band size : 70 kDa