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Products:Neuroscience >> Sensory System >> Visual system
Overview
Product name
Anti-Melanopsin antibody
See all Melanopsin products (5) ...
Description
Rabbit polyclonal to Melanopsin
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Rat
Predicted to work with
Mouse
Immunogen
Synthetic peptide: KMNSPSESRVPSSLTQDPSF, corresponding to amino acids 1-19 of Rat Melanopsin.
KMNSPSESRV PSSLTQDPSF
Positive control
ICC: rat retinal ganglion cells WB: overexpressed melanopsin in HEK293 cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: PBS, 1mg/ml BSA
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab19306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.1 - 0.2 µg/ml.Detects a band of approximately 53,85 kDa.(Endogenous melanopsin is expressed at low levels making it difficult to detect. We recommend running a positive control and conducting experiments on samples overexpressing melanopsin protein.)
IHC-P: Use a concentration of 10 µg/ml
ICC/IF: Use a concentration of 10 µg/ml
Target
Function
Photoreceptor required for regulation of circadian rhythm. Contributes to pupillar reflex and other non-image forming responses to light. May be able to isomerize covalently bound all-trans retinal back to 11-cis retinal.
Tissue specificity
Eye. Expression is restricted within the ganglion and amacrine cell layers of the retina.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
Cellular localization
Membrane. Found in soma, dendrites and proximal part of axons of certain retinal ganglion cells.
Target information above from: UniProt accessionQ9UHM6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Melanopsin antibody
- MGC142118 antibody
- MOP antibody
see all
Database links
- Entrez Gene: 30044 Mouse
- Entrez Gene: 192223 Rat
- SwissProt: Q9QXZ9 Mouse
- SwissProt: Q8R456 Rat
- Unigene: 272060 Mouse
- Unigene: 54303 Rat
Anti-Melanopsin antibody images:
Immunocytochemistry/ Immunofluorescence - Melanopsin antibody (ab19306)
Immunofluorescence and immunocytochemistry staining of melanopsin in rat retinal ganglion cells (RGCs) can be performed using ab19306.
Western blot - Melanopsin antibody (ab19306)
By Western blot, this antibody detects ~53 and ~85 kDa proteins representing overexpressed unglycosylated and glycosylated rat melanopsin from HEK 293 cells.
References for Anti-Melanopsin antibody (ab19306)
This product has been referenced in:
- Boudard DLet al. Loss of photic entrainment at low illuminances in rats with acute photoreceptor degeneration. Eur J Neurosci 30:1527-36 (2009).Read more (PubMed: 19821841) »
See 1 publication for this product
Publishing research using ab19306? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"