Melatonin antibody (ab35137)

Product Name 

Melatonin antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Melatonin

Immunogen 

c.BSA conjugate of 5-Methoxy-DL-tryptophan.

Reacts with 
(species key)

N/A

Specificity 

Reactivity :
Melatonin : 100%
5-Methoxy-DL-Tryptophan : 25%
N-acetyl-Tryptamine : =<0.1%
6-Hydroxy-Melatonin : <0.001%
N-acetyl-Tryptophan : =<0.1%
N-acetyl-Serotonin : =<0.01%

Tested applications 
(see key)

ELISA

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ELISA (hap-KLH, 50%B): 1/40.
ELISA (hap-KLH, EPT): 1/1250.
In competitive ELISA with 250 ng of KLH conjugate of 5-Methoxy-DL-tryptophan coated per well. 25 µl of standard, 25 µl of antibody at final dilution of 1/250.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Neuroscience >> Neurology process >> Circadian Rhythm >> Hormones

Relevance 

Melatonin, the principal hormone of the pineal gland, is also produced by the retina, lens and GI tract. It is naturally synthesized from the amino acid tryptophan (via synthesis of serotonin) by the enzyme 5-hydroxyindole-O-methyltransferase.
Production of melatonin by the pineal gland is under the influence of the suprachiasmatic nucleus of the hypothalamus (SCN -the site of a circadian clock) which receives information from the retina about the daily pattern of light and darkness. Melatonin can alter the timing of mammalian circadian rhythms, as well as regulate the reproductive alterations that occur in response to changes in day length in seasonally breeding mammals.
Melatonin is also an extremely powerful antioxidant, with a particular role in the protection of nuclear and mitochondrial DNA.
Therapeutically there may be many potential uses for melatonin such as in the treatment of various forms of some forms of depression, cancer, HIV, plus other viral diseases. Currently, Melatonin is a popular therapy for jet-lag and disturbances of sleep.

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Ammonium sulfate fractionation.

Form 

Liquid

Concentration 

5.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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