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Products:Signal Transduction >> Protein Trafficking >> Vesicle Transport >> SNAPs & SNAREs
Anti-Membrin antibody [4HAD6 ] (ab13511)
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab13511? Please let us know so that we can cite the reference in this datasheet
ab13511 has been referenced in 2 publications.
- Engel S et al. Mutation of a raft-targeting signal in the transmembrane region retards transport of influenza virus hemagglutinin through the Golgi. FEBS Lett 586:277-82 (2012). ICC/IF. PubMed: 22245151
- Flowerdew SE & Burgoyne RD A VAMP7/Vti1a SNARE complex distinguishes a non-conventional traffic route to the cell surface used by KChIP1 and Kv4 potassium channels. Biochem J 418:529-40 (2009). ICC/IF; Human. PubMed: 19138172
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Immunocytochemistry/ Immunofluorescence - Membrin antibody [4HAD6 ] (ab13511)](/ps/datasheet/Images/13/ab13511/ab13511_1.jpg)
Immunocytochemistry/ Immunofluorescence - Membrin antibody [4HAD6 ] (ab13511)
ab13511 at 1/500 dilution staining human HeLa cells by ICC/IF. HeLa cells, grown on coverslips, were fixed in 3% paraformaldehyde in PBS for 15 minutes. After washing with PBS, the reaction was quenched with 50 mM ammonium chloride for 10 mins. The cells were permeabilized using 0.1 % Triton X100 in PBS, for 2 minutes. After washing 2 times with PBS, the sample was blocked with 2% fish gelatine in PBS. for 1 hour. ab13511 was diluted in the blocking solution, put on the coverslips and incubated for 1 hour on room temperature. After washing 3-4 times with the blocking solution, the sample was incubated in the secondary antibody (Alexa Fluor ® 488 conjugated Goat Anti-Mouse IgG (H+L) diluted in the blocking solution), for 1 hour at room temeperature. The coverslips were washed 6 times with the blocking solution and then mounted on microscope slides.
This image is courtesy of an Abreview submitted by Mr Denis Krndija
![Membrin antibody [4HAD6 ] for ICC/IF in Human (13511)](/ps/Reviews/Images/ab5959_21239.jpg)
Membrin antibody [4HAD6 ] for ICC/IF in Human (13511)
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